Revista: | Brazilian archives of biology and technology |
Base de datos: | PERIÓDICA |
Número de sistema: | 000392748 |
ISSN: | 1516-8913 |
Autores: | Al-Rasheed, Nouf Mohamed1 Fadda, Laila Mohamed1 Al-Rasheed, Nawal Mohamed1 Attia, Hala2 Ali, Hanaa Mahmoud3 El-Agami, Hanaa1 |
Instituciones: | 1King Saud University, Pharmacology Department, Riyadh. Arabia Saudita 2Mansoura University, Department of Biochemistry, Mansoura. Egipto 3National Research Center, El Tabir, Cairo. Egipto |
Año: | 2015 |
Periodo: | Sep-Oct |
Volumen: | 58 |
Número: | 5 |
Paginación: | 741-749 |
País: | Brasil |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Analítico, descriptivo |
Resumen en inglés | The aim of this work was to investigate whether the free radical scavengers, L - arginine (L - arg) and/or carnosine, either alone, or in combination would modulate tissue injury induced by hypoxia by measuring Fischer’s ratio [concentrations of branch ed chain amino acids (BCAAs)/aromatic amino acids]. Decreased Fischer’s ratios and i ncreased malondialdehyde (MDA) led to pathogeneses of many diseases. Rats were injected with sodium nitrite (60 mg/kg) to establish hypoxia. They were treated with L - arg, ( 200 mg/ kg) and/or carnosine (200 mg/ kg) and their combination 24 and 1 h prior to sodium nitrite intoxication. The results revealed that hypoxia significantly decreased hemoglobin, arginine, citrulline and proline and increased sLDH, MDA , ammonia , urea , BCAAs (valine, leucine and isoleucine) and aromatic amino acids (phenylalanine and tyrosine ). The Fischer’s ratio was decreased compared with the control; the administration of the aforementioned antioxidants ameliorated most of the previously altered p arameters. It was concluded that Fischer's ratio was a valuable tool for understanding the pathology of hemic hypoxia, evaluating the degree of the modulatory effect of various natural antioxidants and the synergy between L - arg and carnosine in amelioratin g the effect of sodium nitrite on amino acids pattern. Thus, it could be recommended to administer the combination of L - arg and carnosine in the areas of high altitudes to combat the hazard effect of hypoxia on hemoglobin concentration and MDA level |
Disciplinas: | Química, Medicina |
Palabras clave: | Bioquímica, Química de alimentos, Medicina experimental, Antioxidantes, Aminoácidos, Carnosina, Urea |
Keyword: | Chemistry, Medicine, Biochemistry, Food chemistry, Experimental medicine, Antioxidants, Amino acids, Carnosine, Urea |
Texto completo: | Texto completo (Ver PDF) |