| Revista: | Brazilian archives of biology and technology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000392896 |
| ISSN: | 1516-8913 |
| Autores: | Caron, Angelo Luis1 Castro, Virginia Picanco2 Ansorge, Sven3 Covas, Dimas Tadeu2 Kamen, Amine4 Swiech, Kamilla1 |
| Instituciones: | 1Universidade de Sao Paulo, Faculdade de Ciencias Farmaceuticas, Ribeirao Preto, Sao Paulo. Brasil 2Hemocentro de Ribeirao Preto, Ribeirao Preto, Sao Paulo. Brasil 3National Research Council of Canada, Biotechnology Research Institute, Montreal, Quebec. Canadá 4McGill University, Department of Bioengineering, Montreal, Quebec. Canadá |
| Año: | 2015 |
| Periodo: | Nov-Dic |
| Volumen: | 58 |
| Número: | 6 |
| Paginación: | 923-928 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | Lentiviral vector - mediated gene transfer offers several advantages over other gene delivery vectors when considering gene and cell therapy applications. However, using these therapies in clinical applications involves large-scale vector production in an efficient and cost - effective manner. Here we describe a high yield production of a lentivirus encoding recombinant factor VIII in a scalable and GMP-compliant culture system, based on serum free suspension cultures and transient transfection with an inexpensive reagent, polyethylenimine (PEI), reaching a total viral yield of 2.48x10 8 particles |
| Disciplinas: | Química, Biología |
| Palabras clave: | Química de polímeros, Genética, Virus, Factor VIII, Lentivirus, Polietilenimina, Suero |
| Keyword: | Chemistry, Biology, Polymer chemistry, Genetics, Virus, Factor VIII, Lentivirus, Polyethylenimine, Serum |
| Texto completo: | Texto completo (Ver PDF) |
