Optimization of Endoglucanase Production from a Novel Bacterial Isolate, Arthrobacter sp. HPG166 and Characterization of Its Properties
Document title: Optimization of Endoglucanase Production from a Novel Bacterial Isolate, Arthrobacter sp. HPG166 and Characterization of Its Properties
Journal: Brazilian archives of biology and technology
Database: PERIÓDICA
System number: 000392705
ISSN: 1516-8913
Authors: 1
1
2
1
3
Institutions: 1Chinese Academy of Sciences, Bioenergy Forest Research Center, Hefei, Anhui. China
2Henan University of Science and Technology, College of Food and Bioengineering, Luoyang, Henan. China
3University of Science and Technology of China, School of Life Science, Hefei, Anhui. China
Year:
Season: Sep-Oct
Volumen: 58
Number: 5
Pages: 692-701
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Analítico, descriptivo
English abstract In this study, a potential novel cellulolytic bacterium Arthrobacter sp . HPG166 was isolated from the hindgut of root - feeding larvae Holotrichia parallela . Optimization of fermentation factors for endoglucanase production by Arthrobacter sp . HPG166 was carried out via response surface methodology. Sodium carboxymethylcellulose 1.19% (w/v) and beef extract 0.35% (w/v) were the ideal combination of carbon and nitrogen sources for enzyme production; the optimum temperature and pH for cellulase production were 34°C and pH 8.0 respectively. Under the optimized fermentation conditions, the maximum endoglucanase activity of 1.411 U mL - 1 was obtained. The crude endoglucanase was thermotolerant as it retained 50.31% of its activity after incubation at 70°C for an hour. Metal profile of the enzyme indicated that Mg 2+ and Na + were strong stimulators while Mn 2+ and Co + drastically inhibited its activity. Due to its particular characteristics, this enzyme could have potential for industrial applications
Disciplines: Biología,
Química
Keyword: Bacterias,
Insectos,
Fermentaciones,
Química industrial,
Larvas,
Escarabajos,
Metodología,
Celulasas,
Iones metálicos
Keyword: Biology,
Chemistry,
Bacteria,
Insects,
Fermentation,
Industrial chemistry,
Fermentations,
Larvae,
Scarabs,
Cellulases,
Metal ions
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