| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000392762 |
| ISSN: | 1516-8913 |
| Authors: | Tharachand, C1 Immanuel Selvaraj, C1 Abraham, Z2 |
| Institutions: | 1VIT University, Plant Biotechnology Division, Vellore, Tamil Nadu. India 2National Bureau of Plant Genetic Resources, Regional Station, Kerala. India |
| Year: | 2015 |
| Season: | Sep-Oct |
| Volumen: | 58 |
| Number: | 5 |
| Pages: | 765-772 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | In this work, the genetic relationship among twelve Garcinia cambogia (Gaertn.) Desr. accessions were evaluated using Random Amplified Polymorphic DNA markers. The samples were part of the germplas m collected and maintained at NBPGR Regional station, Thrissur, India. Out of thirty RAPD primers used for screening, seven primers produced a total of 128 polymorphic markers in twelve accessions. The Polymorphic Information Content (PIC) ranged from 0.28 (OPA18) to 0.37 (OPA9) and Marker Index (MI) ranged between 3.61 (OPA12) and 5.93 (OPA3) among the primers used. Jaccard’s coefficient of genetic similarity ranged between 0.07 and 0.64. The dendrogram constructed based on the similarity matrix generated from the molecular and morphological data showed the genetic relationship among the sampled accessions. Mantel matrix test showed a positive correlation (r = 0.49) between the cluster analysis of RAPD data and morphological data. The clustering pattern in the molecular dendrogram and Principle Coordinate Analysis (PCoA) showed that the genotypes were diverse, which was in congruence with the similarity index values and morphological dendrogram. High frequency of similarity values in the range of 0.11 to 0.1 7 suggested the existence of high genetic diversity among the accessions. The high level of genetic diversity among the studied accessions of G.cambogia was also supported by the large variation in the morphological characters observed in the flowers, leaves, fruits and seeds of these sampled accessions. This is the first report for the molecular based genetic diversity studies for these accessions |
| Disciplines: | Agrociencias, Biología, Química |
| Keyword: | Frutales, Genética, Bioquímica, Garcinia cambogia, Diversidad genética, Germoplasma, Coeficiente de Jaccard, Tamarindo, Marcadores moleculares |
| Keyword: | Agricultural sciences, Biology, Chemistry, Fruit trees, Genetics, Biochemistry, Garcinia cambogia, Genetic diversity, Germplasm, Jaccard coefficient, Tamarind, Molecular markers |
| Full text: | Texto completo (Ver PDF) |
