Molecular Cloning and Sequencing of Alkalophilic Cellulosimicrobium cellulans CKMX1 Xylanase Gene Isolated from Mushroom Compost and Characterization of the Gene Product
Document title: Molecular Cloning and Sequencing of Alkalophilic Cellulosimicrobium cellulans CKMX1 Xylanase Gene Isolated from Mushroom Compost and Characterization of the Gene Product
Journal: Brazilian archives of biology and technology
Database: PERIÓDICA
System number: 000392892
ISSN: 1516-8913
Authors: 1
2
1
1
1
Institutions: 1DAV University, Department of Microbiology, Jalandhar, Punjab. India
2Centre for Bioenergy Research, Haryana. India
Year:
Season: Nov-Dic
Volumen: 58
Number: 6
Pages: 913-922
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Analítico, descriptivo
English abstract A xylanolytic bacterium was isolated from mushroom compost by using enrichment technique. Results from the metabolic fingerprinting, whole-cell fatty acids methyl ester analysis and 16S rDNA sequencing suggested the bacterium to be Cellulosimicrobium cellulans CKMX1. Due to the xylanolytic activity of this bacterium, isolation and characterization of the xylanase gene were attempted. A distinct fragment of about 1671 bp was successfully amplified using PCR and cloned into Escherichia coli DH5α. A BLAST search confirmed that the DNA sequence from the amplified fragment was endo - 1, 4 - beta - xylanase, which was a member of glycoside hydrolase family 11. It showed 98% homology with Cellulosimicrobium sp. xylanase gene (Accession no. FJ859907.1) reported from the gut of Eisenia fetida in Korea. In silico physicochemical characterization of amino acid sequence of xylanase showed an open reading frame encoding a 556 amino acid sequence with a molecular weight of 58 kDa and theoretical isolectric po int (pI) of 4.46 was computed using Expasy’s ProtParam server. Secondary and homology based 3D structure of xylanase was analysed using SOPMA and Swiss - Prot software
Disciplines: Biología,
Química
Keyword: Bacterias,
Genética,
Bioquímica,
Xilanasas,
Cellulosimicrobium cellulans,
Escherichia coli,
Clonación,
Genes
Keyword: Biology,
Chemistry,
Bacteria,
Genetics,
Biochemistry,
Xylanases,
Cellulosimicrobium cellulans,
Escherichia coli,
Cloning,
Genes
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