Molecular characterization of arabica and Conilon coffee plants genotypes by SSR and ISSR markers



Document title: Molecular characterization of arabica and Conilon coffee plants genotypes by SSR and ISSR markers
Journal: Brazilian archives of biology and technology
Database: PERIÓDICA
System number: 000388244
ISSN: 1516-8913
Authors: 1
2
3
4

5
Institutions: 1Universidade Federal do Espirito Santo, Programa de Pos-Graduacao em Producao Vegetal, Alegre, Espirito Santo. Brasil
2Universidade Federal do Espirito Santo, Departamento de Farmacia e Nutricao, Alegre, Espirito Santo. Brasil
3Empresa Brasileira de Pesquisa Agropecuaria, Cafe, Vitoria, Espirito Santo. Brasil
4Empresa Brasileira de Pesquisa Agropecuaria, Cafe, Vicosa, Minas Gerais. Brasil
5Universidade Federal do Espirito Santo, Programa de Pos-Graduacao em Genetica e Melhoramento, Alegre, Espirito Santo. Brasil
Year:
Season: Sep-Oct
Volumen: 57
Number: 5
Pages: 728-735
Country: Brasil
Language: Inglés
Document type: Nota breve o noticia
Approach: Experimental
English abstract The molecular characterization of ten genotypes of the Coffea arabica plants and of seven genotypes of C. canephora having interesting features for coffee breeding programs was carried to select the parents for breeding. A total of 40 SSR and 29 ISSR primers were used. The primers generated a total of 331 (307 polymorphic and 24 monomorphic) bands. Analysis of genetic diversity presented dissimilarity intervals ranging from 0.22 to 0.44 between the Conilon genotypes, from 0.02 to 0.28 between the Arabica genotypes, and from 0.49 to 0.60 between the genotypes of the two species in the joint analysis. Four groups were formed: I = genotypes of C. arabica, II = four progenies of C. canephora, Conilon group, and one non defined C. canephora (Conilon or Robusta), III = one progeny of un-defined C. canephora (Conilon or Robusta) and IV = one progeny of C. canephora of Robusta group. The grouping formed was consistent with the origins of each group. High stabilities of the bifurcations were found by bootstrap analysis. The use of molecular markers of the SSR and ISSR types in the diversity study was efficient in distinguishing genotypes between and within C. arabica and C. canephora
Disciplines: Agrociencias
Keyword: Fitotecnia,
Frutales,
Genética,
Café,
Genotipos,
Marcadores moleculares,
Mejoramiento genético,
Coffea arabica,
Coffea canephora
Keyword: Agricultural sciences,
Crop husbandry,
Fruit trees,
Genetics,
Coffee,
Genotypes,
Molecular markers,
Genetic improvement,
Coffea arabica,
Coffea canephora
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