Molecular characterization of arabica and Conilon coffee plants genotypes by SSR and ISSR markers



Título del documento: Molecular characterization of arabica and Conilon coffee plants genotypes by SSR and ISSR markers
Revista: Brazilian archives of biology and technology
Base de datos: PERIÓDICA
Número de sistema: 000388244
ISSN: 1516-8913
Autores: 1
2
3
4

5
Instituciones: 1Universidade Federal do Espirito Santo, Programa de Pos-Graduacao em Producao Vegetal, Alegre, Espirito Santo. Brasil
2Universidade Federal do Espirito Santo, Departamento de Farmacia e Nutricao, Alegre, Espirito Santo. Brasil
3Empresa Brasileira de Pesquisa Agropecuaria, Cafe, Vitoria, Espirito Santo. Brasil
4Empresa Brasileira de Pesquisa Agropecuaria, Cafe, Vicosa, Minas Gerais. Brasil
5Universidade Federal do Espirito Santo, Programa de Pos-Graduacao em Genetica e Melhoramento, Alegre, Espirito Santo. Brasil
Año:
Periodo: Sep-Oct
Volumen: 57
Número: 5
Paginación: 728-735
País: Brasil
Idioma: Inglés
Tipo de documento: Nota breve o noticia
Enfoque: Experimental
Resumen en inglés The molecular characterization of ten genotypes of the Coffea arabica plants and of seven genotypes of C. canephora having interesting features for coffee breeding programs was carried to select the parents for breeding. A total of 40 SSR and 29 ISSR primers were used. The primers generated a total of 331 (307 polymorphic and 24 monomorphic) bands. Analysis of genetic diversity presented dissimilarity intervals ranging from 0.22 to 0.44 between the Conilon genotypes, from 0.02 to 0.28 between the Arabica genotypes, and from 0.49 to 0.60 between the genotypes of the two species in the joint analysis. Four groups were formed: I = genotypes of C. arabica, II = four progenies of C. canephora, Conilon group, and one non defined C. canephora (Conilon or Robusta), III = one progeny of un-defined C. canephora (Conilon or Robusta) and IV = one progeny of C. canephora of Robusta group. The grouping formed was consistent with the origins of each group. High stabilities of the bifurcations were found by bootstrap analysis. The use of molecular markers of the SSR and ISSR types in the diversity study was efficient in distinguishing genotypes between and within C. arabica and C. canephora
Disciplinas: Agrociencias
Palabras clave: Fitotecnia,
Frutales,
Genética,
Café,
Genotipos,
Marcadores moleculares,
Mejoramiento genético,
Coffea arabica,
Coffea canephora
Keyword: Agricultural sciences,
Crop husbandry,
Fruit trees,
Genetics,
Coffee,
Genotypes,
Molecular markers,
Genetic improvement,
Coffea arabica,
Coffea canephora
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