Interference of some organic substances and microorganisms adhered to stainless steel in ATP-bioluminescence measurement



Document title: Interference of some organic substances and microorganisms adhered to stainless steel in ATP-bioluminescence measurement
Journal: Brazilian archives of biology and technology
Database: PERIÓDICA
System number: 000329290
ISSN: 1516-8913
Authors: 1
1
1
1
1
Institutions: 1Universidade Federal de Vicosa, Departamento de Tecnologia de Alimentos, Vicosa, Minas Gerais. Brasil
Year:
Season: May-Jun
Volumen: 51
Number: 3
Pages: 587-593
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Analítico, descriptivo
English abstract Adhesion of organic substances and microorganisms on stainless steel surface was evaluated. The log RLU measurement was affected at a lower or higher degree, by type or concentration of the organic substances (casein, lipid, sucrose and their combinations) adhered to surface. However, if the samples analyzed were from the food processing surfaces, they would have been considered to be under satisfactory hygienic conditions with log RLU < 2.18 (< 150 RLU). The log RLU measurements of Staphylococcus carnosus-adhered surfaces, combined with or without the organic substances, were higher (p<0.05) than those of the Bacillus subtilis spores-adhered surface, regardless of the concentration. The samples taken from the surfaces adhered with the casein, lipid, sucrose and 2.9x101 DMC.cm-2 and 2.9x102 DMC.ml-1 did not have any significant differences (p>0.05) on ATP-bioluminescence measurements. However, the surfaces adhered with 2.9x103 DMC.cm-2 of B. subtilis spores and organic substances showed an increase in the log RLU measurement compared to surfaces containing only the organic substances
Portuguese abstract A adesão de substâncias orgânicas e de microrganismos em cupons de aço inoxidável ASI 304, #4 foi avaliada. A medida de Unidades Relativas de Luz (RLU) foi afetada em menor ou maior nível pelo tipo e concentração das substâncias orgânicas e suas combinações. Entretanto, se as amostras fossem oriundas de superfícies de processamento de alimentos, elas seriam consideradas em boas condições higiênicas pois as medidas de log URL ficaram abaixo de 2,18 log (< 150 URL) com ou sem adição das substâncias orgânicas e independente da concentração estudada, as células vegetativas apresentaram número de log URL para S. carnosus maior (p<0,05) do que para os esporos de B. subtilis. Amostras de superfícies aderidas com caseína, lipídio e sacarose e 2.9x101 CDM.cm-2 ou 2.9x102 CDM.ml-1 não apresentaram diferença significativa (p>0.05) na medida de ATP-bioluminescência. Entretanto, superfícies aderidas com 2.9x103 CDM.cm-2 de esporos de B. subtilis e as substâncias orgânicas mostraram um aumento no log URL comparado com as superfícies contendo somente substâncias orgânicas
Disciplines: Química,
Ciencia y tecnología,
Biología
Keyword: Química de alimentos,
Tecnología,
Bacterias,
Higiene,
Vigilancia,
Medición,
Bioluminiscencia de ATP,
Adherencia,
Acero inoxidable,
Sustancias orgánicas,
Staphylococcus carnosus
Keyword: Chemistry,
Science and technology,
Biology,
Food chemistry,
Technology,
Bacteria,
Hygiene,
Monitoring,
Measurement,
ATP-bioluminescence,
Adhesion,
Stainless steel,
Organic substances,
Staphylococcus carnosus
Full text: Texto completo (Ver HTML)