Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill
Document title: Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill
Journal: Brazilian archives of biology and technology
Database: PERIÓDICA
System number: 000388216
ISSN: 1516-8913
Authors: 1
2
1
1
1
1
Institutions: 1Universidade Federal do Parana, Departamento de Fitotecnia e Fitossanitarismo, Curitiba, Parana. Brasil
2Universidade Federal do Parana, Departamento de Engenharia de Bioprocessos e Biotecnologia, Curitiba, Parana. Brasil
Year:
Season: Sep-Oct
Volumen: 57
Number: 5
Pages: 636-643
Country: Brasil
Language: Inglés
Document type: Nota breve o noticia
Approach: Experimental
English abstract In the present study, the effects of two CaCl2.2H2O levels (440 and 1320 mg L-1) and two subcultures were evaluated on in vitro shoots of Lavandula angustifolia cv. Provence Blue. Ca2+ content of the apical, middle and basal portion of shoots was determined. Increasing CaCl2.2H2O level in the culture medium increased tissue Ca2+ content and decreased hyperhydricity. Shoot-tip necrosis also decreased with 1320 mg L-1 CaCl2.2H2O, but it did not occur in the second subculture. The middle and basal portion had higher Ca2+ content than apical portion. In non-hyperhydric tissues, there were smaller and more juxtaposed cells. Scanning electron microscopy of the leaves demonstrated that trichomes from in vitro leaf surface occurred in smaller quantities
Disciplines: Biología
Keyword: Angiospermas,
Fisiología vegetal,
Propagación vegetativa,
Cultivo de tejidos,
Necrosis,
Brotes,
Hiperhidricidad,
Cloruro de calcio,
Lavandula angustifolia,
Lamiaceae
Keyword: Biology,
Angiosperms,
Plant physiology,
Vegetative propagation,
Tissue culture,
Necrosis,
Shoot tips,
Hyperhydricity,
Calcium chloride,
Lavandula angustifolia,
Lamiaceae
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