Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill
Título del documento: Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill
Revista: Brazilian archives of biology and technology
Base de datos: PERIÓDICA
Número de sistema: 000388216
ISSN: 1516-8913
Autors: 1
2
1
1
1
1
Institucions: 1Universidade Federal do Parana, Departamento de Fitotecnia e Fitossanitarismo, Curitiba, Parana. Brasil
2Universidade Federal do Parana, Departamento de Engenharia de Bioprocessos e Biotecnologia, Curitiba, Parana. Brasil
Any:
Període: Sep-Oct
Volum: 57
Número: 5
Paginació: 636-643
País: Brasil
Idioma: Inglés
Tipo de documento: Nota breve o noticia
Enfoque: Experimental
Resumen en inglés In the present study, the effects of two CaCl2.2H2O levels (440 and 1320 mg L-1) and two subcultures were evaluated on in vitro shoots of Lavandula angustifolia cv. Provence Blue. Ca2+ content of the apical, middle and basal portion of shoots was determined. Increasing CaCl2.2H2O level in the culture medium increased tissue Ca2+ content and decreased hyperhydricity. Shoot-tip necrosis also decreased with 1320 mg L-1 CaCl2.2H2O, but it did not occur in the second subculture. The middle and basal portion had higher Ca2+ content than apical portion. In non-hyperhydric tissues, there were smaller and more juxtaposed cells. Scanning electron microscopy of the leaves demonstrated that trichomes from in vitro leaf surface occurred in smaller quantities
Disciplines Biología
Paraules clau: Angiospermas,
Fisiología vegetal,
Propagación vegetativa,
Cultivo de tejidos,
Necrosis,
Brotes,
Hiperhidricidad,
Cloruro de calcio,
Lavandula angustifolia,
Lamiaceae
Keyword: Biology,
Angiosperms,
Plant physiology,
Vegetative propagation,
Tissue culture,
Necrosis,
Shoot tips,
Hyperhydricity,
Calcium chloride,
Lavandula angustifolia,
Lamiaceae
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