Extraction and Characterization of Polyhydroxybutyrates (PHB) from Bacillus thuringiensisKSADL127 Isolated from Mangrove Environments of Saudi Arabia



Document title: Extraction and Characterization of Polyhydroxybutyrates (PHB) from Bacillus thuringiensisKSADL127 Isolated from Mangrove Environments of Saudi Arabia
Journal: Brazilian archives of biology and technology
Database: PERIÓDICA
System number: 000392768
ISSN: 1516-8913
Authors: 1
1
1
1
Institutions: 1King Saud University, Department of Botany and Microbiology, Ar-Riyad. Arabia Saudita
Year:
Season: Sep-Oct
Volumen: 58
Number: 5
Pages: 781-788
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Analítico, descriptivo
English abstract Polyhydroxybutyrate (PHB) is a renowned biodegradable plastic that do not release any toxins or residues in the environment like petroleum based plastics. In the present study, 50 bacteria isolated from mangrove niche, Saudi Arabia, were screened for maximum PHB production. All the 50 strains showed positive for PHB production, of which one strain showed maximum of 137 mgL - 1 . The most PHB accumulated bacterium was selected and identified as Bacillus thuringiensis KSADL127, based on phenotypic characterization and 16S rRNA sequence analysis. Characterization of extracted PHB was carried out by FT - IR, NMR, UV spectroscopy, DSC, TGA, and LC - MS, which later confirmed the presence of intracellular accumulated polymer and substantiated as PHB
Disciplines: Biología,
Química
Keyword: Bacterias,
Biología acuática,
Química de polímeros,
Química industrial,
Manglares,
Bacillus thuringiensis,
Polihidroxibutirato
Keyword: Biology,
Chemistry,
Aquatic biology,
Bacteria,
Industrial chemistry,
Polymer chemistry,
Mangrove,
Bacillus thuringiensis,
Polyhydroxybutyrate
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