Expression of costimulatory molecules in antigen-activated peritoneal macrophages treated with either ovalbumin or palmitoyl-ova conjugates
Document title: Expression of costimulatory molecules in antigen-activated peritoneal macrophages treated with either ovalbumin or palmitoyl-ova conjugates
Journal: Brazilian archives of biology and technology
Database: PERIÓDICA
System number: 000374045
ISSN: 1516-8913
Authors: 1
2
3
3
Institutions: 1Universidade Federal de Sergipe, Departamento de Educacao em Saude, Lagarto, Sergipe. Brasil
2Universidade Federal de Minas Gerais, Departamento de Morfologia, Belo Horizonte, Minas Gerais. Brasil
3Universidade Federal de Minas Gerais, Departamento de Bioquimica e Imunologia, Belo Horizonte, Minas Gerais. Brasil
Year:
Season: Nov-Dic
Volumen: 56
Number: 6
Pages: 962-970
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract One of the mechanisms by which adjuvants are believed to promote T-cell activation and prevent induction of oral tolerance is by up-regulating the expression of co-stimulatory molecules on antigen presenting cells. Mice treated orally with palmitoyl-ovalbumin conjugates become immunized, while those treated with native ovalbumin (Ova) become tolerant. Cells from the peritoneal cavity of B6D2F1 mice were cultured in the presence of 0.01, or 0.1 mg/100ml of either Ova, or palmitoyl-Ova and tested for the presence of cell markers. PE-conjugated anti-mouse CD80, CD86, and CD11b antibodies as well as biotin-PE were used to stain the antigen-activated peritoneal cells. A significant increase in the expression of CD86 and CD80 was observed following in vitro stimulation with palmitoyl-Ova; additionally, both Ova and palmitoyl-Ova induced the basal expression of CD11b. These findings could be related with the strong T-cell proliferative response induced by palmitoyl-Ova
Disciplines: Biología,
Química
Keyword: Inmunología,
Bioquímica,
Inmunidad celular,
Macrófagos peritoneales,
Activación celular,
Ovalbúmina,
Acido palmítico
Keyword: Biology,
Chemistry,
Immunology,
Biochemistry,
Cell immunity,
Peritoneal macrophages,
Cell activation,
Ovalbumin,
Palmitic acid
Full text: Texto completo (Ver HTML)