| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000391449 |
| ISSN: | 1516-8913 |
| Authors: | Li, Huiying1 Shi, Binlin1 Yan, Sumei1 Zhao, Tianzhang2 Li, Junliang1 Guo, Xiaoyu1 |
| Institutions: | 1Inner Mongolia Agricultural University, College of Animal Science, Hohhot. China 2Wulanchabu Vocational College, Department of Biotechnology, Wulanchabu. China |
| Year: | 2014 |
| Season: | Jul-Ago |
| Volumen: | 57 |
| Number: | 4 |
| Pages: | 466-471 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | An in vitro experiment was conducted to study the effects of chitosan on the secretion of cytokines and expression of inducible nitric oxide synthase mRNA in peritoneal macrophages of broiler chicken. In the experiment, peritoneal macrophages were incubated for 24 h in culture medium supplemented with 0 (control), 40, 80, 160 and 320 µg/mL chitosan. The results showed that chitosan tended to increase quadratically the levels of interleukin-1 (P = 0.093) and interleukin-2 (P = 0.106) in the culture fluid of peritoneal macrophage. Chitosan also significantly enhanced inducible nitric oxide synthase mRNA expression of peritoneal macrophage in a quadratic dose-dependent manner (P < 0.05) and tended to promote quadratically the secretion of nitric oxide (P = 0.053) and inducible nitric oxide synthase (P = 0.157) in peritoneal macrophages. This result implied that one of the mechanisms by which chitosan modulated immune functions in chickens might be chitosan activating expression of inducible nitric oxide synthase and then improving the secretion of nitric oxide |
| Disciplines: | Biología |
| Keyword: | Biología celular, Bioquímica, Macrófagos peritoneales, Pollos, Quitosano, Citocinas, Oxido nítrico sintetasa |
| Keyword: | Biology, Cell biology, Biochemistry, Peritoneal macrophages, Broilers, Chitosan, Cytokines, Nitric oxide synthase |
| Full text: | Texto completo (Ver HTML) |
