Effects of Chitosan on the Secretion of Cytokines and Expression of Inducible Nitric Oxide Synthase mRNA in Peritoneal Macrophages of Broiler Chicken
Título del documento: Effects of Chitosan on the Secretion of Cytokines and Expression of Inducible Nitric Oxide Synthase mRNA in Peritoneal Macrophages of Broiler Chicken
Revista: Brazilian archives of biology and technology
Base de datos: PERIÓDICA
Número de sistema: 000391449
ISSN: 1516-8913
Autores: 1
1
1
2
1
1
Instituciones: 1Inner Mongolia Agricultural University, College of Animal Science, Hohhot. China
2Wulanchabu Vocational College, Department of Biotechnology, Wulanchabu. China
Año:
Periodo: Jul-Ago
Volumen: 57
Número: 4
Paginación: 466-471
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés An in vitro experiment was conducted to study the effects of chitosan on the secretion of cytokines and expression of inducible nitric oxide synthase mRNA in peritoneal macrophages of broiler chicken. In the experiment, peritoneal macrophages were incubated for 24 h in culture medium supplemented with 0 (control), 40, 80, 160 and 320 µg/mL chitosan. The results showed that chitosan tended to increase quadratically the levels of interleukin-1 (P = 0.093) and interleukin-2 (P = 0.106) in the culture fluid of peritoneal macrophage. Chitosan also significantly enhanced inducible nitric oxide synthase mRNA expression of peritoneal macrophage in a quadratic dose-dependent manner (P < 0.05) and tended to promote quadratically the secretion of nitric oxide (P = 0.053) and inducible nitric oxide synthase (P = 0.157) in peritoneal macrophages. This result implied that one of the mechanisms by which chitosan modulated immune functions in chickens might be chitosan activating expression of inducible nitric oxide synthase and then improving the secretion of nitric oxide
Disciplinas: Biología
Palabras clave: Biología celular,
Bioquímica,
Macrófagos peritoneales,
Pollos,
Quitosano,
Citocinas,
Oxido nítrico sintetasa
Keyword: Biology,
Cell biology,
Biochemistry,
Peritoneal macrophages,
Broilers,
Chitosan,
Cytokines,
Nitric oxide synthase
Texto completo: Texto completo (Ver HTML)