Development and Evaluation of an Indirect ELISA: Serological Survey to Detect Specific Antibodies to Bovine Herpesvirus 4



Document title: Development and Evaluation of an Indirect ELISA: Serological Survey to Detect Specific Antibodies to Bovine Herpesvirus 4
Journal: Brazilian archives of biology and technology
Database: PERIÓDICA
System number: 000392746
ISSN: 1516-8913
Authors: 1
2
3
2
4
4
5
Institutions: 1Centro de Diagnostico Marcos Enrietti, Curitiba, Parana. Brasil
2Instituto de Tecnologia do Parana, Curitiba, Parana. Brasil
3Universidade Tuiuti do Parana, Curitiba, Parana. Brasil
4Biogeneses Bago Saude Animal Ltda, Curitiba, Parana. Brasil
5Universidade Federal do Parana, Curitiba, Parana. Brasil
Year:
Season: Sep-Oct
Volumen: 58
Number: 5
Pages: 725-731
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Analítico, descriptivo
English abstract The aim of this work was to develop and evaluate an indirect enzyme - linked immunosorbent assay (ELISA) and made a serological screening for specific antibodies to BoHV - 4. Bovine serum samples were collected from different Brazilian states and evaluated for the presence of antibodies for BoHV - 4 and BoHV - 1. The serological results obtained showed that the indirect ELISA assay could be applied for the detection of specific antibodies for BoHV - 4. The ELISA test allowed concluding that BoHV - 4 is present in bovines in every Brazilian state from which serum samples were collected. The ELISA assay here standardized proved to be useful for the epidemiolo gical studies and showed a positivity range from 1.8 to 66% in Brazil
Disciplines: Medicina veterinaria y zootecnia,
Química
Keyword: Bovinos,
Bioquímica,
Inmunología,
ELISA,
Serología,
Diagnóstico,
Ganado,
Epidemiología
Keyword: Veterinary medicine and animal husbandry,
Chemistry,
Bovines,
Veterinary medicine,
Biochemistry,
Immunology,
ELISA,
Serology,
Diagnosis,
Cattle,
Epidemiology
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