| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000392683 |
| ISSN: | 1516-8913 |
| Authors: | Tan, Dao Xuan1 Tuong, Ho Manh2 Thuy, Vu Thi Thu1 Son, Le Van2 Mau, Chu Hoang1 |
| Institutions: | 1Thai Nguyen University of Education, Vietnam 2Vietnam Academy of Science and Technology, Institute of Biotechnology, Ho Chi Minh City. Vietnam |
| Year: | 2015 |
| Season: | Sep-Oct |
| Volumen: | 58 |
| Number: | 5 |
| Pages: | 651-657 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | This work studied the amplification, cloning and determination of the GmDREB2 gene from the soybean cultivar DT2008 and five Vietnamese local soybean cultivars (DT26, DT51, DVN5, CB, CBD) and designed the vector carrying the structur e containing GmDREB2 gene from cultivar DT2008 (best drought tolerant). The coding region of GmDREB2 gene isolated from six soybean cultivars was 480 nucleotides in length, encoding 159 amino acids. The recombinant structure was designed as 35S - GmDREB2 - c - myc and its expression was analysed in transgenic tobacco plants. Recombinant DREB2 protein was expressed in five transgenic tobacco lines with molecular weights close to 20 kDa. During the drought conditions, the proline accumulation of the transgenic to bacco lines was higher than on wild - type (WT) plants, with the rate from 211.17 to 332.44% after five days of drought stress, and from 262.79 to 466.04% after nine days of drought stress. The two lines, TG2 and TG12 had the highest increase rate. These results provided the basis to generate drought - tolerant soybean plants by GmDREB2 overexpression |
| Disciplines: | Biología, Química, Agrociencias |
| Keyword: | Genética, Bioquímica, Fitotecnia, Resistencia a la sequía, Clones, Variedades, Transferencia génica, Factores de transcripción, Soya |
| Keyword: | Biology, Chemistry, Agricultural sciences, Genetics, Biochemistry, Crop husbandry, Drought resistance, Clones, Cultivars, Gene transfer, Soybean, Transcription factors |
| Full text: | Texto completo (Ver PDF) |
