| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000373464 |
| ISSN: | 1516-8913 |
| Authors: | Gasque, Kellen Cristina da Silva1 Al-Ahj, Luana Polioni1 Oliveira, Rodrigo Cardoso1 Magalhaes, Ana Carolina1 |
| Institutions: | 1Universidade de Sao Paulo, Faculdade de Odontologia de Bauru, Bauru, Sao Paulo. Brasil |
| Year: | 2014 |
| Season: | May-Jun |
| Volumen: | 57 |
| Number: | 3 |
| Pages: | 381-385 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | The aim of this study was to establish the more accurate protocol for fibroblast cell viability using MTT assay. NIH/3T3 fibroblasts were seeded at the following cell densities: 3.125x103; 1.156x104; 3.125x104; 1.156x105 and 3.125x105 cells/cm2. Following 24h of seeding, MTT was added to the wells. After 4h of the MTT addition, different solvents were added to solubilize the formazan crystals: 1) HCl/SDS group- 20% SDS and 0.01 M HCl; 2) EtOH/ HAc group-50% ethanol and 1% acetic acid; 3) DMSO group- 99.5% dimethyl sulfoxide; and 4) PropOH group- 99.5% isopropanol. The absorbance values were measured using a spectrophotometer at 570 nm. The data were analyzed by 2-way ANOVA (p<0.05) and showed that the absorbance average varied according to the number of cells and solvents: HCl/SDS (0 to 0.13), EtOH/HAc (0 to 0.22), DMSO (0.76 to 1.31) and PropOH (0.66 to 1.04). The DMSO and PropOH groups presented the most appropriate protocols for NIH/3T3 fibroblasts cell viability, especially at the density of 1.156x104 cells/cm2 |
| Disciplines: | Biología |
| Keyword: | Biología celular, Cultivo de células, Fibroblastos, Viabilidad de células, Densidad celular, Bioensayos |
| Keyword: | Biology, Cell biology, Cell culture, Fibroblasts, Cell viability, Cell density, Bioassays |
| Full text: | Texto completo (Ver HTML) |
