Aqueous extract of Huang-lian induces apoptosis in lung cancer cells via P53- mediated mitochondrial apoptosis
Document title: Aqueous extract of Huang-lian induces apoptosis in lung cancer cells via P53- mediated mitochondrial apoptosis
Journal: Brazilian archives of biology and technology
Database: PERIÓDICA
System number: 000387699
ISSN: 1516-8913
Authors: 1
1
1
Institutions: 1South China University of Technology, School of Bioscience and Bioengineering, Guangzhou, Guangdong. China
Year:
Season: May-Jun
Volumen: 58
Number: 3
Pages: 353-357
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental
English abstract The current study was designed to evaluate the activity of the aqueous extract of Huang-lian, and the main apoptosis pathway induced by the extracts of Huang-lian was detected on lung cancer. Antiproliferactive activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and TUNEL methods against human lung cancer cells (A549). Huang-lian regulated the Bcl-2 family protein-mediated mitochondrial pathway via p53 and was detected by western blot. It increased the activation of caspase-3 and caspase-3 cleavage increased as the time increased. These results suggested that Huang-lian regulated the Bcl-2 family protein-mediated mitochondrial via p53 pathway, suggesting that Huang-lian should be further investigation as a natural agent for treating and preventing cancer
Disciplines: Medicina,
Química
Keyword: Medicina experimental,
Oncología,
Química farmacéutica,
Extractos vegetales,
Rizoma,
Apoptosis,
Pulmones,
Cáncer,
Coptis chinensis,
Ranunculaceae
Keyword: Medicine,
Chemistry,
Experimental medicine,
Oncology,
Medicinal chemistry,
Exprimental medicine,
Plant extracts,
Rhizome,
Apoptosis,
Lungs,
Cancer,
Coptis chinensis,
Ranunculaceae
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