Aqueous extract of Huang-lian induces apoptosis in lung cancer cells via P53- mediated mitochondrial apoptosis
Título del documento: Aqueous extract of Huang-lian induces apoptosis in lung cancer cells via P53- mediated mitochondrial apoptosis
Revista: Brazilian archives of biology and technology
Base de datos: PERIÓDICA
Número de sistema: 000387699
ISSN: 1516-8913
Autors: 1
1
1
Institucions: 1South China University of Technology, School of Bioscience and Bioengineering, Guangzhou, Guangdong. China
Any:
Període: May-Jun
Volum: 58
Número: 3
Paginació: 353-357
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental
Resumen en inglés The current study was designed to evaluate the activity of the aqueous extract of Huang-lian, and the main apoptosis pathway induced by the extracts of Huang-lian was detected on lung cancer. Antiproliferactive activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and TUNEL methods against human lung cancer cells (A549). Huang-lian regulated the Bcl-2 family protein-mediated mitochondrial pathway via p53 and was detected by western blot. It increased the activation of caspase-3 and caspase-3 cleavage increased as the time increased. These results suggested that Huang-lian regulated the Bcl-2 family protein-mediated mitochondrial via p53 pathway, suggesting that Huang-lian should be further investigation as a natural agent for treating and preventing cancer
Disciplines Medicina,
Química
Paraules clau: Medicina experimental,
Oncología,
Química farmacéutica,
Extractos vegetales,
Rizoma,
Apoptosis,
Pulmones,
Cáncer,
Coptis chinensis,
Ranunculaceae
Keyword: Medicine,
Chemistry,
Experimental medicine,
Oncology,
Medicinal chemistry,
Exprimental medicine,
Plant extracts,
Rhizome,
Apoptosis,
Lungs,
Cancer,
Coptis chinensis,
Ranunculaceae
Text complet: Texto completo (Ver HTML)