| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000392904 |
| ISSN: | 1516-8913 |
| Authors: | Ceylan, Ozgur1 Alıc, Halime2 |
| Institutions: | 1Mugla University, Mugla. Turquía 2Mugla Sitki Kocman University, Mugla. Turquía |
| Year: | 2015 |
| Season: | Nov-Dic |
| Volumen: | 58 |
| Number: | 6 |
| Pages: | 935-943 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | This is the first study to investigate the antibiofilm, antioxidant and antimutagenic activities and phenolic compounds of A llium orientale. Antimicrobial activity of ethanolic extracts of A . orientale was determined by a broth microdilution method. Antibiofilm activity was evaluated by microplate biofilm assay. The antioxidant activity was determined using three complementary assays; namely, DPPH scavenging, β - carotene - linoleic acid, and total phenolic compounds assays. Phenolic compounds were evaluated by reverse - phase high - performance liquid chromatography. The antimutagenic effect of extracts was analyzed by the Ames test. In RP - HPLC analysis, (+) - catechin, apigenin and caffeic acid were identified as major phenolic compounds in the aerial parts of A . orientale. The aerial parts extract possessed the highest total phenolic content (120.979 ± 1.05 mg gallic acid equivalent/g), which were in good correlation with its significant DPPH (IC50 42.18 ± 1.68 mg/mL) and lipid peroxidation (89.98 ± 0.69% at 10 mg/ mL concentration) capacities. A. orientale exhibited potent antimicrobial activity against the organisms tested with MICs ranging from 3.125 to 25 mg/ mL. Escherichia coli biofilm formation was inhibited maximum by the aerial parts extract to an extent of 68.51%. The strongest antimutagenic activity was observed at 2.5 mg/pla te concentration of aerial parts extract against S almonella typhimurium TA98. These results suggested that the ethanolic extract of the aerial parts of A . orientale could become useful supplement for pharmaceutical products as a new antioxidant, antibiofilm and antimutagenic agent |
| Disciplines: | Química |
| Keyword: | Bioquímica, Fitoquímica, Química farmacéutica, Allium orientale, Antibióticos, Antioxidantes, Actividad antimutagénica, Compuestos fenólicos |
| Keyword: | Chemistry, Biochemistry, Medicinal chemistry, Phytochemistry, Allium orientale, Antibiotics, Antioxidants, Antimutagenic activity, Phenolic compounds |
| Full text: | Texto completo (Ver PDF) |
