| Journal: | Biotecnología vegetal |
| Database: | PERIÓDICA |
| System number: | 000347057 |
| ISSN: | 1609-1841 |
| Authors: | Collado, Raúl1 García, Lourdes R1 Angenon, Gert1 Torres, Dámaris1 Romero, Carlos1 Bermúdez-Caraballoso, Idalmis1 Veitía, Novisel1 Ramírez, Miriam1 |
| Institutions: | 1Universidad Central "Marta Abreu" de Las Villas, Instituto de Biotecnología de las Plantas, Santa Clara, Villa Clara. Cuba |
| Year: | 2008 |
| Season: | Abr-Jun |
| Volumen: | 8 |
| Number: | 2 |
| Pages: | 81-86 |
| Country: | Cuba |
| Language: | Español |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| Spanish abstract | La principal limitante para la transformación genética de Phaseolus vulgaris L. está relacionada con las dificultades para su regeneración in vitro. Para transformar genéticamente uno de los cultivares de mayor importancia en Cuba se requiere desarrollar previamente un protocolo de regeneración in vitro vía organogénesis indirecta. Es por ello, que este trabajo se realizó con el objetivo de lograr la formación de brotes a partir de callos en Phaseolus vulgaris L. cv. CIAP 7247. Se emplearon como material vegetal semillas maduras cosechadas en una casa de cultivos protegidos. En la formación de callos se determinó el efecto del tipo de explante. Para lograr la formación de brotes a partir de callos, se probaron cuatro concentraciones de 6-bencilaminopurina (0.5, 1.0, 2.0, 2.5 mg.l -1 ) y un tratamiento control sin reguladores de crecimiento. Transcurridos 45 días de cultivo, se evaluó el número de brotes formados en 0.5g de masa fresca de callo. El mayor porcentaje (86%) de explantes que formaron callo se observó en los cotiledones, seguido por los segmentos de eje embriogénico con nudo cotiledonal (57%) y por los cotiledones con eje embriogénico (38%). Sin embargo, solo se formaron brotes en los callos que crecieron a partir de cotiledones con eje embriogénico. Con la adición de 2.0 mg.l -1 de 6-BAP al medio de cultivo de formación de brotes el número de brotes observados en 0.5 g de callos se incrementó 2.2 veces. Se demostró que el tipo de explante tuvo un efecto determinante en la regeneración |
| English abstract | The main difficulty that limits the genetic transformation in Phaseolus vulgaris L. is the low responsiveness for in vitro regeneration. The development of an in vitro regeneration via organogenesis protocol is needed in order to achieve the genetic transformation in one of the most important cultivar of this legume in Cuba. Therefore, the aim of this work was to obtain shoots formation from morphogenetic calli in Phaseolus vulgaris L. cv. CIAP 7247. Matures seeds harvested in greenhouse were used as plant material. Different explants were studied for callus formation (cotyledon, cotyledon with a segment of embryonic axis and segment of embryonic axis with cotyledonal nude). Number of explants with callus formation was evaluated after 28 days. The percentage was also determined. Formed calli were classified according to their consistency and structure in compacts-nodular and soft – spongy. Three concentration of 6-bencylaminopurine (0.5, 1.0 and 2.0 mg.l -1 ) and control treatment without growth regulator were studied to achieve shoot induction and formation from calli. Number of shoots formed on 0.5 g of callus fresh mass, after 45 days of culture, was evaluated. The highest percentage (86%) of explants with callus formation was determinate on cotyledons. Segments of embryonic axis with cotyledonal nude produced 57%, and cotyledons with embryonic axis 38%. However, shoots were only formed on calli grown from cotyledons with embryonic axis. The addition of 6-BAP 2.0 mg.l -1 to shoot formation medium increased in 2.2 the number of shoots observed on 0.5 g of calli. It was demonstrated that the kind of explant had a determinant effect on calli regeneration |
| Disciplines: | Biología, Agrociencias |
| Keyword: | Biología celular, Leguminosas, Biotecnología, Frijol, Phaseolus vulgaris, Plantas transgénicas, Callos embriogénicos, Cultivo de tejidos, Explantes, Regeneración |
| Keyword: | Biology, Agricultural sciences, Cell biology, Legumes, Biotechnology, Bean, Phaseolus vulgaris, Transgenic plants, Embryogenic callus, Tissue culture, Explants, Regeneration |
| Full text: | Texto completo (Ver PDF) |
