| Journal: | Biocell |
| Database: | PERIÓDICA |
| System number: | 000343490 |
| ISSN: | 0327-9545 |
| Authors: | Cavicchia, Juan Carlos1 Fóscolo, Mabel1 Ibañez, Jorge1 Lillig, Christopher2 Capani, Francisco3 |
| Institutions: | 1Universidad Nacional de Cuyo, Instituto de Histología y Embriología "Dr. Mario H. Burgos", Mendoza. Argentina 2Phillips-Universitat Marburg, Institute for Clinical Cytobiology and Cytopathology, Marburg, Hessen. Alemania 3Universidad de Buenos Aires, Instituto de Investigaciones Cardiológicas "Prof. Dr. Alberto C. Taquini", Buenos Aires. Argentina |
| Year: | 2011 |
| Volumen: | 35 |
| Number: | 3 |
| Pages: | 81-89 |
| Country: | Argentina |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Junctional devices in Sertoli cells conform the blood-testis barrier and play a key role in maturation and differentiation of germ cells. The spacial distribution of ectoplasmic specializations of Sertoli cells was studied by β-actin immunolabelling, using laser confocal and transmission electron microscopy. For confocal microscopy, β-actin immunolabelling of ectoplasmic specializations was studied over the background of either prosaposin or glutaredoxin immunolabelling of the Sertoli cytoplasm. Labelling was found near the basal lamina, surrounding early spermatocytes (presumably in leptotene-zygotene) or at one of two levels in the seminiferous epithelium: (1) around deep infoldings of the Sertoli cell cytoplasm, in tubular stages before spermiation, and (2) in the superficial part of the seminiferous epithelium, in tubular stages after or during spermiation. For transmission electron microscopy, β-actin immunolabelling of ectoplasmic specializations was also used. Ectoplasmic specializations were found at two different levels of the seminiferous epithelium. We also used freeze fracture to analyze the characteristics of tubulo-bulbar complexes, a known component of apical ectoplasmic specializations. Also, these different approaches allowed us to study the complex arrangement of the actin cytoskeleton of Sertoli cells branches, which surround germ cells in different stages of the spermatogenic cycle. Our results show a consistent labelling for β-actin before, during and after the release of spermatozoa in the tubular lumen (spermiation) suggesting a significant role of the actin network in spermatic cell differentiation. In conclusion, significant interrelations among the β-actin network, the junctional complexes of the blood-testis barrier and the ectoplasmic specializations were detected at different stages of the seminiferous cycle |
| Disciplines: | Medicina, Biología |
| Keyword: | Biología celular, Células de Sertoli, Beta actina, Filamentos, Epitelio seminífero, Células germinales, Diferenciación celular |
| Keyword: | Medicine, Biology, Cell biology, Sertoli cells, Beta actin, Filaments, Seminiferous epithelium, Germ cells, Cell differentiation |
| Full text: | Texto completo (Ver PDF) |
