| Journal: | Biocell |
| Database: | PERIÓDICA |
| System number: | 000330406 |
| ISSN: | 0327-9545 |
| Authors: | Cavicchia, Juan Carlos1 Guembe, Gustavo1 Fóscolo, Mabel1 |
| Institutions: | 1Universidad Nacional de Cuyo, Instituto de Histología y Embriología "Dr. Mario H. Burgos", Mendoza. Argentina |
| Year: | 2010 |
| Season: | Ago |
| Volumen: | 34 |
| Number: | 2 |
| Pages: | 81-89 |
| Country: | Argentina |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | In a previous paper we described a pronounced increase of apoptotic nuclei in rat corpus luteum of pregnancy whose programmed chromatin degeneration was induced by the progesterone antagonist mifepristone. Those observations encouraged us to study the apoptotic nuclear membrane during pregnancy and after parturition and pup removal, by using a freeze-fracture technique which allows us to observe ‘en face’ the nuclear envelop and also permits nuclear pore counting. This study was complemented with the TUNEL assay (TdT-mediated dUTP nick-end labelling). Changes in nuclear pores during pregnancy begin with an intense reduction in number but still showing an even distribution on the nuclear membrane, never forming aggregations sharply separated from pore-free areas, which are characteristic of other apoptotic models. Electron microscopy of thin-sections shows, coincidently with findings in the freeze-fracture replicas, a moderately irregular aggregation of marginal heterochromatin condensations. After nuclear fragmentation and micronuclear formation, pores behave in the usual manner in other apoptotic models, i.e., mainly showing migrations of nuclear pores toward the chromatin-free areas. The present results support the hypothesis that nuclear pore complexes are dynamic structures, which permit their migration toward nuclear membrane areas devoid of chromatin aggregations that might block the nucleocytoplasmic transport in such areas |
| Disciplines: | Biología |
| Keyword: | Biología celular, Cuerpo lúteo, Poros nucleares, Membrana nuclear, Transporte núcleo-citoplasma, Apoptosis |
| Keyword: | Biology, Cell biology, Corpus luteum, Nuclear pores, Nuclear membrane, Nucleocytoplasmic transport, Apoptosis |
| Full text: | Texto completo (Ver PDF) |
