| Journal: | Biocell |
| Database: | PERIÓDICA |
| System number: | 000321418 |
| ISSN: | 0327-9545 |
| Authors: | Rodríguez, Andrea P Tsujigiwa, Hidetsugu Gunduz, Mehmet Cengiz, Beyhan Nagai, Noriyuki Tamamura, Ryo Borkosky, Silvia S Takagi, Tohru Inoue, Miho Nagatsuka, Hitoshi1 |
| Institutions: | 1Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama. Japón |
| Year: | 2009 |
| Season: | Abr |
| Volumen: | 33 |
| Number: | 1 |
| Pages: | 39-47 |
| Country: | Argentina |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Progenitor cells play an important biological role in tooth and bone formation, and previous analyses during bone and dentine induction have indicated that they may be a good alternative for tissue engineering. Thus, to clarify the influence of the microenvironment on protein and gene expression, MDPC- 23 cells (mouse dental papilla cell line) and KUSA/A1 cells (bone marrow stromal cell line) were used, both in vitro cell culture and in intra-abdominal diffusion chambers implanted in 4-week-old male immunodefficient mice (SCID mice). Our results indicate that KUSA/A1 cells differentiated into osteoblast-like cells and induced bone tissue inside the chamber, whereas, MDPC-23 showed odontoblast-like characteristics but with a low ability to induce dentin formation. This study shows that MDPC-23 cells are especial cells, which possess morphological and functional characteristics of odontoblast-like cells expressing dentin sialophosphoprotein in vivo. In contrast, dentin sialophosphoprotein gene and protein expression was not detected in both cell lines in vitro. The intra-abdominal diffusion chamber appears as an interesting experimental model for studying phenotypic expression of dental pulp cells in vivo |
| Disciplines: | Biología, Medicina |
| Keyword: | Biología celular, Genética, Medicina experimental, Células progenitoras, Diferenciación celular, Expresión génica, Análisis inmunohistoquímico |
| Keyword: | Biology, Medicine, Cell biology, Genetics, Experimental medicine, Progenitor cells, Cell differentiation, Gene expression, Immunohistochemistry |
| Full text: | Texto completo (Ver PDF) |
