| Journal: | Biocell |
| Database: | PERIÓDICA |
| System number: | 000395130 |
| ISSN: | 0327-9545 |
| Authors: | Pronsato, Lucia1 La Colla, Anabela1 Ronda, Ana Carolina1 Milanesi, Lorena1 Boland, Ricardo1 Vasconsuelo, Andrea1 |
| Institutions: | 1Universidad Nacional del Sur, Departamento de Biología, Bioquímica y Farmacia, Bahía Blanca, Buenos Aires. Argentina |
| Year: | 2013 |
| Season: | Abr |
| Volumen: | 37 |
| Number: | 1 |
| Pages: | 1-9 |
| Country: | Argentina |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (<20) or high (>60) passage numbers (identified as l- C2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H 2 O 2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H 2 O 2 induction of apoptosis. We propose serially cultured C2C12 cells as a refractory model to H 2 O 2 -induced apoptosis. In addition, the data obtained in this work suggest that mtDNA is required for apoptotic cell death in skeletal muscle C2C12 cells |
| Disciplines: | Biología |
| Keyword: | Anatomía e histología, Biología celular, Genética, Líneas celulares, Músculo esquelético, Apoptosis, ADN mitocondrial, Cultivo de células |
| Keyword: | Biology, Anatomy and histology, Cell biology, Genetics, Cell lines, Skeletal muscle, Apoptosis, Mitochondrial DNA, Cell culture |
| Full text: | Texto completo (Ver PDF) |
