| Journal: | Biocell |
| Database: | PERIÓDICA |
| System number: | 000335534 |
| ISSN: | 0327-9545 |
| Authors: | Talia, Paola1 Greizerstein, Eduardo J1 Hopp, H. Esteban2 Paniego, Norma1 Poggio, Lidia2 Heinz, Ruth A2 |
| Institutions: | 1Instituto Nacional de Tecnología Agropecuaria, Instituto de Biotecnología, Hurlingham, Buenos Aires. Argentina 2Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Buenos Aires. Argentina |
| Year: | 2011 |
| Volumen: | 35 |
| Number: | 1 |
| Pages: | 19-28 |
| Country: | Argentina |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Bacterial artificial chromosome - fluorescence in situ hybridization (BAC-FISH) and cyclingprimed in situ labeling (C-PRINS) techniques were evaluated for integration of physical and genetic maps of sunflower (Helianthus annuus L.). Single-site SSR markers were selected from three linkage groups of a high-density sunflower genetic map. This selection was based on previously identified QTL associated to S. sclerotiorum. These markers were used to select BACs contaning single copy sequences for BAC-FISH aplication. Blocking of highly dispersed repetitive sunflower sequences reduced unspecific hybridization, and allowed the detection of specific signals for BACs containing SSR markers HA4222 and HA2600, anchored to LG 16 and LG 10, respectively. Single-site FISH signal detection was optimized by adjusting the relative quantity and quality of unlabelled repetitive sequences present in the blocking DNA. The SSR marker ORS1247 anchored to the LG 17 was detected by C-PRINS, which yielded fluorescence signals that were specific and intense. This progress in localizing single-copy sequences using BAC-FISH and indirect CPRINS strategies in sunflower will facilitate the integration of genetic and physical maps, allowing the identification of chromosomes containing key genes and/or QTL associated to agronomic important traits in sunflower |
| Disciplines: | Biología |
| Keyword: | Angiospermas, Genética, Mapeo genético, Secuencia génica, Marcadores moleculares, Girasol, Helianthus annuus, Compositae |
| Keyword: | Biology, Angiosperms, Genetics, Genetic mapping, Gene sequence, Molecular markers, Sunflower, Helianthus annuus, Compositae |
| Full text: | Texto completo (Ver PDF) |
