| Journal: | Biocell |
| Database: | PERIÓDICA |
| System number: | 000345422 |
| ISSN: | 0327-9545 |
| Authors: | Surenciski, M.R1 Flachsland, E.A1 Terada, G1 Mroginski, L.A1 Rey, H.Y1 |
| Institutions: | 1Instituto de Botánica del Nordeste, Facultad de Ciencias Agrarias, Corrientes. Argentina |
| Year: | 2012 |
| Season: | Abr |
| Volumen: | 36 |
| Number: | 1 |
| Pages: | 31-36 |
| Country: | Argentina |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| English abstract | The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen for 12 h. Cryopreserved beads were thawed at 30°C for 1 min, rehydrated using the same liquid mediums [0.75 M sucrose (24 h), 0.5 M sucrose (24 h), 0.25 M sucrose (48 h) and 0.15 M sucrose (24 h)] and cultivated in half strength Murashige & Skoog medium (1962) with the addition of 2 g/L activated charcoal. Sixty four percent of seeds survived and developed into acclimatized plants after being cryopreserved. In this work, the encapsulation-dehydration technique was employed for first time in Cyrtopodium hatschbachii |
| Disciplines: | Biología |
| Keyword: | Biología celular, Botánica, Biotecnología, Cyrtopodium hatschbachii, Crioconservación, Orchidaceae, Semillas, Nitrógeno líquido, Alginato de calcio, Sucrosa |
| Keyword: | Biology, Botany, Cell biology, Biotechnology, Cyrtopodium hatschbachii, Cryopreservation, Seeds, Orchidaceae, Liquid nitrogen, Calcium alginate, Sucrose |
| Full text: | Texto completo (Ver PDF) |
