A study of chlorophyll-like and phycobilin pigments in the C endosymbiont of the apple- snail Pomacea canaliculata
Título del documento: A study of chlorophyll-like and phycobilin pigments in the C endosymbiont of the apple- snail Pomacea canaliculata
Revista: Biocell
Base de datos: PERIÓDICA
Número de sistema: 000352016
ISSN: 0327-9545
Autores: 1
1
2
1
1
Instituciones: 1Universidad Nacional de Cuyo, Facultad de Ciencias Médicas, Mendoza. Argentina
2Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Buenos Aires. Argentina
Año:
Volumen: 36
Número: 2
Paginación: 47-55
País: Argentina
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés Pigments present in the brown-greenish C morph of an intracellular endosymbiont of Pomacea canaliculata were investigated. Acetone extracts of the endosymbiotic corpuscles showed an absorption spectrum similar to that of chlorophylls. Three fractions obtained from silica gel column chromatography of the acetone extracts (CI, CII and CIII), were studied by positive ion fast atom bombardment-mass spectrometry (FAB-MS) and hydrogen-nuclear magnetic resonance (H-NMR). Results indicated the presence of (1) a sterol in the yellow colored CI fraction; (2) a mixture of pheophorbides a and b in the major green fraction, CII; and (3) a modified pheophorbide a in the smaller green fraction, CIII. Aqueous extracts of the C endosymbiont did not show evidence of the occurrence of C-phycocyanin, allophycocyanin or phycoerithrin (light absorption, fluorescence emission, and electrophoresis of the protein moieties) while cyanobacterial cells (Nostoc sp.) showed evidence of C-phycocyanin and allophycocyanin. The possible phylogenetic and functional significance of the pigments present in the C endosymbiont is discussed
Disciplinas: Biología
Palabras clave: Bacterias,
Moluscos,
Endosimbiontes,
Pigmentos fotosintéticos,
Ficobilina,
Cianobacterias,
Pomacea canaliculata,
Ampullariidae
Keyword: Biology,
Bacteria,
Molluscs,
Endosymbionts,
Photosynthetic pigments,
Phycobilin,
Cyanobacteria,
Pomacea canaliculata,
Ampullariidae
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