| Journal: | Annals of hepatology |
| Database: | PERIÓDICA |
| System number: | 000411378 |
| ISSN: | 1665-2681 |
| Authors: | Attallah, Abdelfattah M1 El-Far, Mohamed2 Abdel Malak, Camelia A3 Omran, Mohamed M4 Farid, Khaled5 Hussien, Mostafa A6 Albannan, Mohamed S1 Attallah, Ahmed A1 Elbendary, Mohamed S1 Elbesh, Dalia A1 Elmenier, Noha A1 Abdallah, Mohamed O1 |
| Institutions: | 1Biotechnology Research Center, Research & Development Department, New Damietta. Egipto 2Mansoura University, Faculty of Science, Mansoura. Egipto 3Damietta University, Faculty of Science, New Damietta. Egipto 4Helwan University, Faculty of Science, El Cairo. Egipto 5Mansoura University, Faculty of Medicine, Mansoura. Egipto 6Port-Said University, Faculty of Science, Port-Said. Egipto |
| Year: | 2015 |
| Season: | Mar-Abr |
| Volumen: | 14 |
| Number: | 2 |
| Pages: | 225-233 |
| Country: | México |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | The assessment of liver fibrosis provides useful information not only for diagnosis but also for therapeutic decision. This study was concerned with determining the levels of collagen III and its degrading enzyme matrix metalloproteinase-1 (MMP-1) as direct and complementary markers for liver fibrosis staging. Results. A total of 269 chronic hepatitis C patients constituted this study. Western blotting was used for identifying collagen III and MMP-1 in serum samples. As a result, collagen III and MMP-1 were identified, respectively, at 70 and 245 kDa using their respective mono-specific antibodies. These two markers were quantified in sera of patients using ELISA. Next, Fibro-check was constructed combining collagen III and MMP-1 together with other indirect markers which reflect alteration in hepatic functions that proved useful to stage liver fibrosis. Fibro-check produced area under the receiver-operating characteristic curve (AUC) 0.91 and 0.83 for significant (F2-F4) and cirrhosis (F4), respectively. Additionally, we estimated the performance of Fibro-check in comparison with aspartate to platelet ratio index (APRI) and fibrosis index. Fibro-check seems to be more efficient than both of them. Fibro-check was then applied to the validation study to test its accuracy and reproducibility showing AUCs 0.90 for F2-F4 and 0.86 for F4. Conclusions. Fibro-check combining ‘direct’ and ‘indirect’ markers using a mathematical formula may improve the staging of liver fibrosis with a high degree of accuracy and seems more efficient than APRI and Fibrosis index in this group of Egyptian patients |
| Disciplines: | Medicina |
| Keyword: | Diagnóstico, Gastroenterología, Hepatitis crónica, Hepatitis C, Fibrosis hepática, Estadificación, Metaloproteinasas, Marcadores de pronóstico |
| Keyword: | Medicine, Diagnosis, Gastroenterology, Chronic hepatitis, Hepatitis C, Liver fibrosis, Staging, Metalloproteinases, Prognosis markers |
| Full text: | Texto completo (Ver PDF) |
