Journal: | Annals of hepatology |
Database: | PERIÓDICA |
System number: | 000416123 |
ISSN: | 1665-2681 |
Authors: | Almajhdi, Fahad N1 Al-Qudari, Ahmed Y1 Hussain, Zahid1 |
Institutions: | 1King Saud University, College of Science, Riyadh. Arabia Saudita |
Year: | 2013 |
Volumen: | 12 |
Number: | 3 |
Pages: | 408-415 |
Country: | México |
Language: | Inglés |
Document type: | Artículo |
Approach: | Experimental, analítico |
English abstract | This study investigated how HBV replication and host immune response are effected by reduced expression of TGF-β1 and HBx. Material and methods. Short interfering RNA (siRNA) knockdown technology has been used to examine the role of TGF-β1 in hepatitis B virus replication. The siTGF-β1 has been transfected along with 1.3mer HBV x-null to investigate the knockdown effect of TGF-β1 on HBV replication and host immune factors. Results. In this study, we found that diminished expression of TGF-β1 and increased expression of HBx enhances HBV replication several folds. The differential expression of TGF-β1 and HBx also stimulated transcriptional viral replicative intermediate (pgRNA) and secretion of core and ‘e’ antigen at translational level. Consequently, several cytokines such as IL-2, IL-8 and chemokine monocyte-chemoattractant protein (MCP-1) were increased significantly in response to stimulation of HBV replication. In contrast, TNF-α and RANTES mRNA expression increased insignificantly in response to enhanced HBV replication. Conclusions. We concluded that reduced expression of TGF-β1 together with HBx expression stimulate HBV replication and immune response, although the underlying mechanism of stimulation most likely differs |
Disciplines: | Medicina |
Keyword: | Gastroenterología, Inmunología, Terapéutica y rehabilitación, Virus de la hepatitis C, Factor de crecimiento transformante, Hepatocitos, Replicación viral, Respuesta inmune |
Keyword: | Gastroenterology, Immunology, Hepatitis B virus, Transforming growth factor, Hepatocytes, Viral replication, Immune response |
Full text: | Texto completo (Ver PDF) |