Differential expression of transforming growth factor-β and HBx enhances hepatitis B virus replication and augments host immune cytokines and chemokines
Título del documento: Differential expression of transforming growth factor-β and HBx enhances hepatitis B virus replication and augments host immune cytokines and chemokines
Revista: Annals of hepatology
Base de datos: PERIÓDICA
Número de sistema: 000416123
ISSN: 1665-2681
Autores: 1
1
1
Instituciones: 1King Saud University, College of Science, Riyadh. Arabia Saudita
Año:
Volumen: 12
Número: 3
Paginación: 408-415
País: México
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, analítico
Resumen en inglés This study investigated how HBV replication and host immune response are effected by reduced expression of TGF-β1 and HBx. Material and methods. Short interfering RNA (siRNA) knockdown technology has been used to examine the role of TGF-β1 in hepatitis B virus replication. The siTGF-β1 has been transfected along with 1.3mer HBV x-null to investigate the knockdown effect of TGF-β1 on HBV replication and host immune factors. Results. In this study, we found that diminished expression of TGF-β1 and increased expression of HBx enhances HBV replication several folds. The differential expression of TGF-β1 and HBx also stimulated transcriptional viral replicative intermediate (pgRNA) and secretion of core and ‘e’ antigen at translational level. Consequently, several cytokines such as IL-2, IL-8 and chemokine monocyte-chemoattractant protein (MCP-1) were increased significantly in response to stimulation of HBV replication. In contrast, TNF-α and RANTES mRNA expression increased insignificantly in response to enhanced HBV replication. Conclusions. We concluded that reduced expression of TGF-β1 together with HBx expression stimulate HBV replication and immune response, although the underlying mechanism of stimulation most likely differs
Disciplinas: Medicina
Palabras clave: Gastroenterología,
Inmunología,
Terapéutica y rehabilitación,
Virus de la hepatitis C,
Factor de crecimiento transformante,
Hepatocitos,
Replicación viral,
Respuesta inmune
Keyword: Gastroenterology,
Immunology,
Hepatitis B virus,
Transforming growth factor,
Hepatocytes,
Viral replication,
Immune response
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