| Journal: | Anais da Academia Brasileira de Ciencias |
| Database: | PERIÓDICA |
| System number: | 000436074 |
| ISSN: | 0001-3765 |
| Authors: | Yurre, Ainhoa R. de1 Martins, Eduarda G.L1 Lopez Alarcon, Micaela1 Cabral, Bruno1 Vera, Narendra1 Lopes, Jarlene A1 Galina, Antonio2 Takiya, Christina M1 Lindoso, Rafael S1 Vieyra, Adalberto1 Sáenz, Oscar C3 Medei, Emiliano1 |
| Institutions: | 1Universidade Federal do Rio de Janeiro, Instituto de Biofisica Carlos Chagas Filho, Rio de Janeiro. Brasil 2Universidade Federal do Rio de Janeiro, Instituto de Bioquimica Medica Leopoldo de Meis, Rio de Janeiro. Brasil 3Universidad del País Vasco, Facultad de Farmacia, Vitoria-Gasteiz. España |
| Year: | 2020 |
| Volumen: | 92 |
| Number: | 2 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Type 2 diabetes mellitus (T2DM) is associated with an increase of premature appearance of several disorders such as cardiac complications. Thus, we test the hypothesis that a combination of a high fat diet (HFD) and low doses of streptozotocin (STZ) recapitulate a suitable mice model of T2DM to study the cardiac mitochondrial disturbances induced by this disease. Animals were divided in 2 groups: the T2DM group was given a HFD and injected with 2 low doses of STZ, while the CNTRL group was given a standard chow and a buffer solution. The combination of HFD and STZ recapitulate the T2DM metabolic profile showing higher blood glucose levels in T2DM mice when compared to CNTRL, and also, insulin resistance. The kidney structure/function was preserved. Regarding cardiac mitochondrial function, in all phosphorylative states, the cardiac mitochondria from T2DM mice presented reduced oxygen fluxes when compared to CNTRL mice. Also, mitochondria from T2DM mice showed decreased citrate synthase activity and lower protein content of mitochondrial complexes. Our results show that in this non-obese T2DM model, which recapitulates the classical metabolic alterations, mitochondrial function is impaired and provides a useful model to deepen study the mechanisms underlying these alterations |
| Disciplines: | Medicina |
| Keyword: | Endocrinología, Medicina experimental, Diabetes, Mitocondria, Corazón, Modelos animales |
| Keyword: | Endocrinology, Experimental medicine, Diabetes, Heart, Mitochondria, Animal models |
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