| Journal: | Anais da Academia Brasileira de Ciencias |
| Database: | PERIÓDICA |
| System number: | 000435627 |
| ISSN: | 0001-3765 |
| Authors: | Chagas, Kristhiano1 Cipriano, Jamile L.D2 Lopes, José Carlos3 Schmildt, Edilson R4 Otoni, Wagner C1 Alexandre, Rodrigo S3 |
| Institutions: | 1Universidade Federal de Vicosa, Instituto de Biotecnologia Aplicada a Agropecuaria, Vicosa, Minas Gerais. Brasil 2Instituto Federal de Minas Gerais, Sabara, Minas Gerais. Brasil 3Universidade Federal do Espirito Santo, Centro de Ciencias Agrarias e Engenharias, Alegre, Espirito Santo. Brasil 4Universidade Federal do Espirito Santo, Centro Universitario Norte do Espirito Santo, Sao Mateus, Espirito Santo. Brasil |
| Year: | 2018 |
| Season: | Dic |
| Volumen: | 90 |
| Number: | 4 |
| Pages: | 3433-3447 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | This study evaluated the effect of osmoregulators and carbohydrates on the maturation and germination of somatic embryos of papaya ‘Golden THB’. Cotyledon explants from papaya seedlings germinated in vitro on basal MS medium were cultured on somatic embryogenesis induction medium (IM) containing MS salts, myo-inositol, sucrose, agar and p-chlorophenoxyacetic acid. After 50 days, embryogenic calli were transferred onto maturation media (MM) for 45 additional days. For experiment 1, a MS-based medium supplemented with abscisic acid, activated charcoal and concentrations of PEG 6000 (0; 40; 50; 60 and 70 g L-1) was used, whereas for experiment 2 malt extract concentrations (0; 0.1; 0.2; 0.3 and 0.4 g L-1) were assessed. The normal cotyledonary somatic embryos produced in experiment 2 were transferred to the germination medium (GM). The GM consisted of full-strength MS medium, sucrose, agar and was supplemented with myo-inositol at varying concentrations (0; 0.275; 0.55 and 0.825 mM). The PEG concentrations tested impaired the maturation of ‘Golden THB’ papaya somatic embryos. The MM, supplemented with malt extract at 0.153 g L-1, promoted the greatest development of normal somatic embryos (18.28 SE calli-1), that is, two cotyledonary leaves produced 36.56 SE calli-1. The supplementation with 0.45 mM myo-inositol provided the highest germination percentage (47.42%) and conversion to emblings |
| Disciplines: | Agrociencias, Biología |
| Keyword: | Fisiología vegetal, Frutales, Embriogénesis somática, Medios de cultivo, Osmorreguladores, Extracto de malta, Mio-inositol, Polietilenglicol, Carica papaya |
| Keyword: | Plant physiology, Fruit trees, Somatic embryogenesis, Culture media, Osmoregulators, Malt extract, Myo-inositol, Polyethylene glycol, Carica papaya |
| Full text: | https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000703433&lng=en&nrm=iso&tlng=en |
