| Journal: | Anais da Academia Brasileira de Ciencias |
| Database: | PERIÓDICA |
| System number: | 000435884 |
| ISSN: | 0001-3765 |
| Authors: | Meira, Filipe S1 Luis, Zanderluce G2 Cardoso, Inae Marie A.S3 Scherwinski Pereira, Jonny E3 |
| Institutions: | 1Universidade de Brasilia, Programa de Pos-Graduacao em Biotecnologia e Biodiversidade, Brasilia, Distrito Federal. Brasil 2Universidade Federal do Sul e Sudeste do Para, Instituto de Estudo em Saude e Biologicas, Maraba, Para. Brasil 3Empresa Brasileira de Pesquisa Agropecuaria, Recursos Geneticos e Biotecnologia, Brasilia, Distrito Federal. Brasil |
| Year: | 2020 |
| Volumen: | 92 |
| Number: | 3 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | A somatic embryogenesis protocol was developed from the immature leaves of adult plants of the macaw palm. Leaf explants from different regions of the palm heart were used for callus initiation in a modified Y3 medium, supplemented with 2,4-D or Picloram at 450 μM. Calli were separated from the leaf explants at 6-, 9- and 12-month periods and transferred to a fresh culture medium of the same composition. They were multiplied for up to 120 days. Reduced concentrations of 2,4-D and Picloram were used to differentiate somatic embryos. They were then germinated in a medium without plant growth regulators. Morphological and anatomical analyses were conducted at different stages of the embryogenic process. The best results for callus induction were achieved by Picloram, when explants were maintained for up to 9 months on culture medium (64.9%). The farthest portions of the apical meristem were those that provided the biggest calli formation. The formation of the somatic embryos was observed from the calli multiplication phase. Reduction in concentrations of growth regulators failed to promote the formation of complete plants. Picloram at 450 μM promotes high callogenesis in leaf tissues of macaw palm, with a potential for somatic embryo formation |
| Disciplines: | Biología, Agrociencias |
| Keyword: | Fisiología vegetal, Cultivo de tejidos, Embriogénesis somática, Plantas leñosas, Explantes foliares, Acrocomia aculeata, Arecaceae |
| Keyword: | Plant physiology, Tissue culture, Somatic embryogenesis, Woody plants, Leaf explants, Acrocomia aculeata, Arecaceae |
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