| Journal: | Anais da Academia Brasileira de Ciencias |
| Database: | PERIÓDICA |
| System number: | 000436032 |
| ISSN: | 0001-3765 |
| Authors: | Sehnem, Nicole T1 Machado, Angela S1 Matte, Carla R1 Morais-Junior, Marcos Antonio de2 Ayub, Marco Antonio Z1 |
| Institutions: | 1Universidade Federal do Rio Grande do Sul, Departamento de Tecnologia de Alimentos, Porto Alegre, Rio Grande do Sul. Brasil 2Universidade Federal de Pernambuco, Departamento de Genetica, Recife, Pernambuco. Brasil |
| Year: | 2020 |
| Volumen: | 92 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | The aims of this work were to improve cell tolerance towards high concentrations of furfural and 5-hydroxymethylfurfural (HMF) of an osmotolerant strain of Wickerhamomyces anomalus by means of evolutionary engineering, and to determine its ethanol production under stress conditions. Cells were grown in the presence of furfural, HMF, either isolated or in combination, and under high osmotic pressure conditions. The most toxic condition for the parental strain was the combination of both furans, under which it was unable to grow and to produce ethanol. However, the tolerant adapted strain achieved a yield of ethanol of 0.43 g g-1glucose in the presence of furfural and HMF, showing an alcohol dehydrogenase activity of 0.68 mU mg protein-1. For this strain, osmotic pressure, did not affect its growth rate. These results suggest that W. anomalus WA-HF5.5strain shows potential to be used in second-generation ethanol production systems |
| Disciplines: | Biología, Química |
| Keyword: | Microbiología, Biotecnología, Hidrolisados lignocelúlosicos, Presión osmótica, Furaldehídos, Bioproducción, Etanol, Wickerhamomyces anomalus |
| Keyword: | Microbiology, Biotechnology, Lignocellulosic hydrolysates, Osmotic pressure, Furaldehydes, Bioproduction, Ethanol, Wickerhamomyces anomalus |
| Full text: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
