Phenolic content and antioxidant capacity of infusions herbs: Optimization of phenolic extraction and HPLC-DAD method
Document title: Phenolic content and antioxidant capacity of infusions herbs: Optimization of phenolic extraction and HPLC-DAD method
Journal: Anais da Academia Brasileira de Ciencias
Database: PERIÓDICA
System number: 000435951
ISSN: 0001-3765
Authors: 1
1
Institutions: 1Universidade do Estado da Bahia, Departamento de Ciencias Exatas e da Terra, Salvador, Bahia. Brasil
2Universidade Federal da Bahia, Departamento de Quimica Analitica, Salvador, Bahia. Brasil
Year:
Volumen: 92
Number: 3
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Aplicado, descriptivo
English abstract The aim of this work was to investigate the phenolic content and antioxidant capacity of infusions of commercial herb samples (fennel, anise, peppermint, lemon grass and lemon balm) popularly consumed in Brazil. The infusion preparation for phenolic extraction was optimized using multivariate planning. Spectrophotometric methods were used to determine in vitro antioxidant activity and total phenolic and flavonoid content. Peppermint infusions had higher phenolic content and antioxidant potential. It was developed and validated a method by HPLC-DAD for the determination of caffeine, phenolic acids and flavonoids and applied for the analysis of the composition of the infusions. Higher concentrations were obtained for chlorogenic and p-coumaric phenolic acids and for flavonoids rutin and catechin. Principal Components Analysis and Hierarchical Cluster Analysis were applied for the comparative evaluation of the phenolic composition of the infusions. The multivariate analyzes indicate that the phenolic profile for the samples of the same species tend to present greater similarities in relation to other herbs and one of the analyzed samples, commercialized as anise, does not belong to the P. anisum species
Disciplines: Química
Keyword: Fitoquímica,
Hierbas aromáticas,
Infusiones,
Compuestos fenólicos,
Extracción química,
Cromatografía líquida de alta resolución (HPLC),
Capacidad antioxidante
Keyword: Phytochemistry,
Aromatic herbs,
Infusions,
Phenolic compounds,
Chemical extraction,
High performance liquid chromatography (HPLC),
Antioxidant capacity
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