Inhibitory effects on the production of inflammatory mediators and reactive oxygen species by Mori folium in lipopolysaccharide-stimulated macrophages and zebrafish
Document title: Inhibitory effects on the production of inflammatory mediators and reactive oxygen species by Mori folium in lipopolysaccharide-stimulated macrophages and zebrafish
Journal: Anais da Academia Brasileira de Ciencias
Database: PERIÓDICA
System number: 000408987
ISSN: 0001-3765
Authors: 1
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Institutions: 1Dongeui University, Department of Biochemistry, Busan. Corea del Sur
2Dongeui University, Anti-Aging Research Center, Busan. Corea del Sur
3Korea Institute of Oriental Medicine, Daejeon, Chungcheong del Sur. Corea del Sur
4Marine Bio-industry Development Center, Busan. Corea del Sur
5Jeju National University, Laboratory of Immunology, Jeju. Corea del Sur
6Dongeui University, Department of Molecular Biology, Busan. Corea del Sur
7Dongeui University, Department of Food and Nutrition, Busan. Corea del Sur
8Daejeon University, Liver and Immunology Research Center, Daejeon, Chungcheong del Sur. Corea del Sur
Year:
Season: May
Volumen: 89
Pages: 661-674
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Analítico, descriptivo
English abstract Mori folium, the leaf of Morus alba L. (Moraceae), has been traditionally used for various medicinal purposes from ancient times to the present. In this study, we examined the effects of water extract of Mori folium (WEMF) on the production of inflammatory mediators, such as nitric oxide (NO) and prostaglandin E 2 (PGE 2 ), and reactive oxygen species (ROS) in lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophages. Our data indicated that WEMF significantly suppressed the secretion of NO and PGE 2 in RAW 264.7 macrophages without any significant cytotoxicity. The protective effects were accompanied by a marked reduction in their regulatory gene expression at the transcription level. WEMF attenuated LPS- induced intracellular ROS production in RAW 264.7 macrophages. It inhibited the nuclear translocation of the nuclear factor-kappa B p65 subunit and the activation of mitogen-activated protein kinases in LPS- treated RAW 264.7 macrophages. Furthermore, WEMF reduced LPS-induced NO production and ROS accumulation in zebrafish. Although more efforts are needed to fully understand the critical role of WEMF in the inhibition of inflammation, the findings of the present study may provide insights into the approaches for Mori folium as a potential therapeutic agent for inflammatory and antioxidant disorders
Disciplines: Química,
Medicina
Keyword: Fitoquímica,
Terapéutica y rehabilitación,
Peces,
Morus alba,
Mori folium,
Inflamación,
Especies reactivas de oxígeno (ROS),
Macrófagos,
Pez cebra
Keyword: Chemistry,
Medicine,
Phytochemistry,
Therapeutics and rehabilitation,
Fish,
Morus alba,
Mori folium,
Inflammation,
Reactive oxygen species (ROS),
Macrophages,
Zebra fish
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