| Journal: | Anais da Academia Brasileira de Ciencias |
| Database: | PERIÓDICA |
| System number: | 000436319 |
| ISSN: | 0001-3765 |
| Authors: | Cechim, Giovana1 Chies, José A.B1 |
| Institutions: | 1Universidade Federal do Rio Grande do Sul, Instituto de Biociencias, Porto Alegre, Rio Grande do Sul. Brasil |
| Year: | 2019 |
| Volumen: | 91 |
| Number: | 4 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Dendritic cells (DCs) initiate and shape both innate and adaptive immune responses. They are specialized in antigen presentation to naive T cells, thereby orchestrating the T cell immune responses. Human peripheral blood and tissues contain several subsets of phenotypically and functionally distinct DCs, which promote interactions between the external environment and lymphoid organs. Because of the difficulty in purifying these cells, in vitro studies only became more frequent when Frederica Sallusto and Antonio Lanzavecchia developed a method to generate DCs from blood monocytes in vitro. Nowadays a wide range of biotechnological innovations has allowed the study of DCs and their precursors in the most diverse situations faced by the immune system. As a result of such studies, monocyte-derived dendritic cells (MDDC) are presently used in clinical protocols for the treatment of a variety of diseases, including cancer and human immunodeficiency virus infection. We summarize recent advances in the understanding of methodologies and inputs used in protocols to differentiate DCs from blood monocytes in vitro |
| Disciplines: | Biología |
| Keyword: | Biología celular, Inmunología, Cultivo in vitro, Células dendríticas, Citocinas, Diferenciación celular, Monocitos |
| Keyword: | Cell biology, Immunology, In vitro culture, Cytokines, Dendritic cells, Cell differentiation, Monocytes |
| Full text: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
