| Journal: | Anais da Academia Brasileira de Ciencias |
| Database: | PERIÓDICA |
| System number: | 000424445 |
| ISSN: | 0001-3765 |
| Authors: | Vilela, Thais C1 Leffa, Daniela D1 Damiani, Adriani P1 Dmazio, Daiane dal col1 Manenti, Aline V1 Carvalho, Tiago Jose G2 Ramlov, Fernanda2 Amaral, Patricia A3 Andrade, Vanessa M. de1 |
| Institutions: | 1Universidade do Sul de Santa Catarina, Unidade de Ciencias da Saude, Criciuma, Santa Catarina. Brasil 2Universidade Federal de Santa Catarina, Departamento de Botanica, Florianopolis, Santa Catarina. Brasil 3Universidade do Sul de Santa Catarina, Laboratorio de Plantas Medicinais, Criciuma, Santa Catarina. Brasil |
| Year: | 2018 |
| Season: | Sep |
| Volumen: | 90 |
| Number: | 3 |
| Pages: | 3165-3174 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Hibiscus acetosella was shown to exert beneficial effects in humans and animal models however, the effects of this plant on DNA are unknown. The aim of this study was to determine the antigenotoxic and antimutagenic effects of H. acetosella extracts on alkylating agent methyl methanesulfonate (MMS) in vivo in mice. Initially, we performed analysis of phenolic compounds in extracts of H. acetosella by high-performance liquid chromatography (HPLC). Next, mice were divided into 8 groups and treated with distilled water or plant extract (0.1 ml/10 g) by gavage for 15 days, followed by intraperitoneal (ip) administration of saline solution or MMS (40 mg/Kg b.w) on day 16. Caffeic acid, following by gallic acid, gallocatechin, coumaric acid, and 3,4-dihydroxybenzoic acid were found to be present in extracts of H. acetosella leaves. In peripheral blood analysis of groups receiving pretreatment with H. acetosella at doses of 50 or 100 mg/kg plus MMS decreased DNA damage as evidenced by comet assay and Micronucleus assays relative to MMS alone. These results suggested that H. acetosella extracts exerted protective effects dose dependent against genotoxicity and mutagenicity induced by alkylating agents |
| Disciplines: | Medicina, Química, Biología |
| Keyword: | Angiospermas, Fitoquímica, Química farmacéutica, Plantas medicinales, Extractos vegetales, Actividad antimutagénica, Antigenotoxicidad, Hibiscus acetosella, Malvaceae |
| Keyword: | Angiosperms, Phytochemistry, Medicinal chemistry, Medicinal plants, Plant extracts, Antimutagenic activity, Antigenotoxicity, Hibiscus acetosella, Malvaceae |
| Full text: | Texto completo (Ver HTML) |
