Ethanolic extract of Croton blanchetianus Ball induces mitochondrial defects in Leishmania amazonensis promastigotes
Document title: Ethanolic extract of Croton blanchetianus Ball induces mitochondrial defects in Leishmania amazonensis promastigotes
Journal: Anais da Academia Brasileira de Ciencias
Database: PERIÓDICA
System number: 000435994
ISSN: 0001-3765
Authors: 1
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2
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Institutions: 1Universidade Federal de Sergipe, Sao Cristovao, Sergipe. Brasil
2Instituto Oswaldo Cruz, Rio de Janeiro. Brasil
Year:
Volumen: 92
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract Leishmaniasis is a neglected disease caused by Leishmania. Chemotherapy remains the mainstay for leishmaniasis control; however, available drugs fail to provide a parasitological cure, and are associated with high toxicity. Natural products are promising leads for the development of novel chemotherapeutics against leishmaniasis. This work investigated the leishmanicidal properties of ethanolic extract of Croton blanchetianus (EECb) on Leishmania infantum and Leishmania amazonensis, and found that EECb, rich in terpenic compounds, was active against promastigote and amastigote forms of both Leishmania species. Leishmania infantum promastigotes and amastigotes presented IC50 values of 208.6 and 8.8 μg/mL, respectively, whereas Leishmania amazonensis promastigotes and amastigotes presented IC50 values of 73.6 and 3.1 μg/mL, respectively. Promastigotes exposed to EECb (100 µg/mL) had their body cellular volume reduced and altered to a round shape, and the flagellum was duplicated, suggesting that EECb may interfere with the process of cytokinesis, which could be the cause of the decline in the parasite multiplication rate. Regarding possible EECb targets, a marked depolarization of the mitochondrial membrane potential was observed. No cytotoxic effects of EECb were observed in murine macrophages at concentrations below 60 µg/mL, and the CC50 obtained was 83.8 µg/mL. Thus, the present results indicated that EECb had effective and selective effects against Leishmania infantum and Leishmania amazonensis, and that these effects appeared to be mediated by mitochondrial dysfunction
Disciplines: Biología,
Química
Keyword: Fitoquímica,
Protozoarios,
Extractos vegetales,
Extracto etanólico,
Toxicidad,
Metabolismo mitocondrial,
Croton blanchetianus,
Euphorbiaceae,
Leishmania amazonensi,
Leishmania infantum
Keyword: Phytochemistry,
Protozoa,
Plant extracts,
Ethanolic extract,
Toxicity,
Mitochondrial metabolism,
Croton blanchetianus,
Euphorbiaceae,
Leishmania amazonensi,
Leishmania infantum
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