Effect of dietary NiCl2 on the cell cycle of cecal tonsil in the chicken broiler
Título del documento: Effect of dietary NiCl2 on the cell cycle of cecal tonsil in the chicken broiler
Revista: Anais da Academia Brasileira de Ciencias
Base de datos: PERIÓDICA
Número de sistema: 000436004
ISSN: 0001-3765
Autores: 1
1
3
1
Instituciones: 1China West Normal University, College of Life Science, Nanchong, Sichuan. China
2Ministry of Education, Key Laboratory of Southwest China Wildlife Resources Conservation, Nanchong, Sichuan. China
3Sichuan Agricultural University, College of Veterinary Medicine, Chengdu, Sichuan. China
Año:
Volumen: 92
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Aplicado, descriptivo
Resumen en inglés Although the effects of nickel chloride (NiCl2) on the immune system have long been recognized, little is known about the effects of nickel (II) on the cell cycle and related signaling events in immune organs, such as cecal tonsil, a key immune organ of chicken. In the present study, we investigated the effect of NiCl2 on the cell cycle of cecal tonsil. The cell cycle was detected by the methods of flow cytometry (FCM), quantitative real-time PCR (qRT-PCR) and immunohistochemistry (IHC). The results showed that dietary NiCl2 in excess of 300 mg/kg caused the G2/M cell cycle arrest and the reduction of cell proportion at S phase of the cecal tonsil. The G2/M cell cycle arrest was accompanied by the up-regulation of p53, p21 protein expression and mRNA expression, and down-regulation of cyclinB and proliferating cell nuclear antigen (PCNA) protein expression and mRNA expression. The data suggested that the cells’ (mainly the T lymphocytes) proliferation in the cecal tonsil was inhibited by the high dietary NiCl2
Disciplinas: Medicina veterinaria y zootecnia
Palabras clave: Aves de corral,
Nutrición animal,
Pollos de engorda,
Cloruro de níquel,
Ciclo celular,
Amígdala cecal
Keyword: Poultry,
Animal nutrition,
Broilers,
Nickel chloride,
Cell cycle,
Cecal tonsil
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