| Journal: | Anais da Academia Brasileira de Ciencias |
| Database: | PERIÓDICA |
| System number: | 000420629 |
| ISSN: | 0001-3765 |
| Authors: | Londe, Luciana C.N1 Vendrame, Wagner A2 Sanaei, Massy2 Oliveira, Alexandre B. de3 |
| Institutions: | 1Empresa de Pesquisa Agropecuária de Minas Gerais, Campo Experimental do Gorutuba, Nova Porteirinha, Minas Gerais. Brasil 2University of Florida, Tropical Research and Education Center, Homestead, Florida. Estados Unidos de América 3Universidade Federal do Ceara, Departamento de Ciencias Agrarias, Fortaleza, Ceara. Brasil |
| Year: | 2018 |
| Season: | Mar |
| Volumen: | 90 |
| Number: | 1 |
| Pages: | 495-508 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | The preservation of banana genetic material is usually performed through seedlings. However, most banana cultivars do not produce seed and are propagated vegetatively. Therefore, cryopreservation is a feasible technique that allows the preservation of banana genotypes indefinitely. For the success of cryopreservation protocols, the selection of cryoprotectants and pre-freezing techniques are important factor. Therefore, the objective of this study was to verify the effects of different cryoprotectants with and without 1% phloroglucinol and pre-cooling periods on the development of a protocol for cryopreservation of in vitro rhizomes ofMusa accuminata(AAA) cv Grand Naine banana. The addition of 1% phloroglucinol to the cryoprotective solutions, such as PVS2 enhanced recovery of cryopreserved banana rhizomes. In addition, pre-cooling of explants in ice for 3 hours in PVS2 + 1% of phloroglucinol allowed efficient cryopreservation of banana rhizomes, followed by successful recovery and regeneration of in vitro shoots of banana cv Grand Naine |
| Disciplines: | Agrociencias |
| Keyword: | Fitotecnia, Frutales, Criopreservación, Crioprotectores, Rizomas, Plátano, Musa acuminata |
| Keyword: | Crop husbandry, Fruit trees, Cryopreservation, Cryoprotectants, Rhizomes, Banana, Musa acuminata |
| Full text: | Texto completo (Ver HTML) |
