Cryopreservation of banana’s cv Grand Naine in vitro rhizomes



Título del documento: Cryopreservation of banana’s cv Grand Naine in vitro rhizomes
Revista: Anais da Academia Brasileira de Ciencias
Base de datos: PERIÓDICA
Número de sistema: 000420629
ISSN: 0001-3765
Autores: 1
2
2
3
Instituciones: 1Empresa de Pesquisa Agropecuária de Minas Gerais, Campo Experimental do Gorutuba, Nova Porteirinha, Minas Gerais. Brasil
2University of Florida, Tropical Research and Education Center, Homestead, Florida. Estados Unidos de América
3Universidade Federal do Ceara, Departamento de Ciencias Agrarias, Fortaleza, Ceara. Brasil
Año:
Periodo: Mar
Volumen: 90
Número: 1
Paginación: 495-508
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Analítico, descriptivo
Resumen en inglés The preservation of banana genetic material is usually performed through seedlings. However, most banana cultivars do not produce seed and are propagated vegetatively. Therefore, cryopreservation is a feasible technique that allows the preservation of banana genotypes indefinitely. For the success of cryopreservation protocols, the selection of cryoprotectants and pre-freezing techniques are important factor. Therefore, the objective of this study was to verify the effects of different cryoprotectants with and without 1% phloroglucinol and pre-cooling periods on the development of a protocol for cryopreservation of in vitro rhizomes ofMusa accuminata(AAA) cv Grand Naine banana. The addition of 1% phloroglucinol to the cryoprotective solutions, such as PVS2 enhanced recovery of cryopreserved banana rhizomes. In addition, pre-cooling of explants in ice for 3 hours in PVS2 + 1% of phloroglucinol allowed efficient cryopreservation of banana rhizomes, followed by successful recovery and regeneration of in vitro shoots of banana cv Grand Naine
Disciplinas: Agrociencias
Palabras clave: Fitotecnia,
Frutales,
Criopreservación,
Crioprotectores,
Rizomas,
Plátano,
Musa acuminata
Keyword: Crop husbandry,
Fruit trees,
Cryopreservation,
Cryoprotectants,
Rhizomes,
Banana,
Musa acuminata
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