Comparison of Bovine Small Antral Follicle Development in Two- and Three-Dimensional Culture Systems
Document title: Comparison of Bovine Small Antral Follicle Development in Two- and Three-Dimensional Culture Systems
Journal: Anais da Academia Brasileira de Ciencias
Database: PERIÓDICA
System number: 000435988
ISSN: 0001-3765
Authors: 1
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Institutions: 1Northwest A&F University, College of Veterinary Medicine, Yangling, Shaanxi. China
Year:
Volumen: 92
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract To compare the effects of two-(2D, microplate) and three-dimensional (3D, alginate) culture systems on the in vitro growth of small antral follicles in cattle, individual follicles were separately cultured in the two culture systems for 8 days. Half of the culture medium was replaced by fresh medium every 2 days; the former medium was used to assess the amount of follicular hormone secretion using ELISA. Individual follicle morphology, diameter, and survival rate were recorded every alternate day. The results showed that in 4 days, there was no significant difference between the two systems, except that the growth rate of follicles in 2D system was relatively faster. After 4 days, estradiol concentration in 3D system was higher than that in 2D system. However, progesterone concentration was lower than that in the 2D system. The survival rate and oocyte quality of follicles in 2D system were significantly lower than those in 3D system on day 8. The follicle diameter slightly increased (30-60 μm) in the entire process. Taken together, for in vitro culture of follicles within 4 days, the 2D culture system is more suitable. However, when the culture duration is >4 days, the 3D culture system is more suitable
Disciplines: Medicina veterinaria y zootecnia
Keyword: Reproducción y genética animal,
Bovinos,
Foliculos antrales,
Cultivo de tejidos,
Medios de cultivo
Keyword: Animal reproduction and genetics,
Bovines,
Antral follicles,
Tissue culture,
Culture media
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