| Journal: | Anais da Academia Brasileira de Ciencias |
| Database: | PERIÓDICA |
| System number: | 000435883 |
| ISSN: | 0001-3765 |
| Authors: | Grunennvaldt, Renata Lúcia1 Degenhardt Goldbach, Juliana2 Brooks, Peter3 Tomasi, Jéssica de Cássia1 Hansel, Fabrício Augusto2 Tran, Trong3 Gomes, Erik N4 Deschamps, Cícero1 |
| Institutions: | 1Universidade Federal do Parana, Departamento de Fitotecnia, Curitiba, Parana. Brasil 2Centro Nacional de Pesquisa Forestal, Colombo, Parana. Brasil 3University of the Sunshine Coast, Sippy Downs, Queensland. Australia 4The State University of New Jersey, New Brunswick, New Jersey. Estados Unidos de América |
| Year: | 2020 |
| Volumen: | 92 |
| Number: | 3 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Aplicado, descriptivo |
| English abstract | Ilex paraguariensis (yerba mate) is a native species from South America and is a rich source of bioactive compounds. There is a lack of research efforts on the phytochemical investigation of callus culture from this species. In the present study, an effort was made to optimize callus culture conditions and to identify secondary compounds. Calli were induced from 10 genotypes using leaf explants and the best genotype was selected to evaluate the effects of cytokinin types and concentrations on callus induction and biomass accumulation. The best genotype and cytokinin treatment were used to conduct one last experiment with sucrose concentrations in culture media and its effects on calli biomass, antioxidant activity and secondary compounds accumulation. Callus initiation was genotype dependent, and the 6-156-6 line had the best response. Zeatin supplemented medium showed higher callus induction rate (82%) and higher biomass accumulation after 120 days (328.2 mg). Higher biomass and secondary compounds accumulation were observed for calli on 3% sucrose medium. Antioxidant activity was not affected by sucrose concentrations. Yerba mate callus culture allowed the accumulation of chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, theobromine and caffeine |
| Disciplines: | Agrociencias, Biología |
| Keyword: | Angiospermas, Fisiología vegetal, Propagación vegetativa, Reguladores del crecimiento, Actividad antioxidante, Producción de biomasa, Sacarosa, Ilex paraguariensis, Aquifoliaceae |
| Keyword: | Angiosperms, Plant physiology, Vegetative propagation, Antioxidant activity, Biomass production, Growth regulators, Sucrose, Ilex paraguariensis, Aquifoliaceae |
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