Ameliorative potential of Butea monosperma on chronic constriction injury of sciatic nerve induced neuropathic pain in rats
Document title: Ameliorative potential of Butea monosperma on chronic constriction injury of sciatic nerve induced neuropathic pain in rats
Journal: Anais da Academia Brasileira de Ciencias
Database: PERIÓDICA
System number: 000366654
ISSN: 0001-3765
Authors: 1
2
1
3
3
Institutions: 1Shanmugha Arts, Science, Technology and Research Academy, School of Chemical and Biotechnology, Thanjavur, Tamil Nadu. India
2Sankaralingam Bhuvaneshwari College of Pharmacy, Department of Pharmaceutical Sciences and Research, Thiruthangal, Tamil Nadu. India
3Punjabi University, Department of Pharmaceutical Sciences and Drug Research, Patiala, Punjab. India
Year:
Season: Dic
Volumen: 84
Number: 4
Pages: 1091-1104
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract The present study was designed to investigate the ameliorative role of ethanolic extract from leaves of Butea monosperma in chronic constriction injury (CCI) of sciatic nerve induced neuropathic pain in rats. Hot plate, acetone drop, paw pressure, Von Frey hair and tail immersion tests were performed to assess the degree of thermal hyperalgesia, cold chemical allodynia, mechanical hyperalgesia & allodynia in the left hind paw and tail thermal hyperalgesia. Further on, thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH) and total calcium levels were estimated to assess the biochemical changes in the sciatic nerve tissue. Histopathological changes were also observed in the sciatic nerve tissue. Ethanolic extract of Butea monosperma leaves and pregabalin (serving as positive control) were administered for 14 consecutive days starting from the day of surgery. CCI resulted in significant changes in behavioural and biochemical parameters. Pretreatment of Butea monosperma attenuated CCI induced development of behavioural, biochemical and histopathological alterations in a dose dependent manner, which is comparable to that of pregabalin pretreated group. These findings may be attributed to its potential anti-oxidative, neuroprotective and calcium channel modulatory actions of Butea monosperma
Portuguese abstract O presente trabalho visou investigar o papel do extrato etanólico de folhas de Butea monosperma no alívio da dor neuropática pela injúria de constrição crônica (CCI) do nervo ciático induzida em ratos. Placa quente, gota de acetona, pressão na pata, testes de imersão de pelo e cauda de Von Frey foram utilizados para acessar o grau de hiperalgesia térmica, alodinia química fria, hiperalgesia mecânica e alodinia na pata trazeira esquerda e hiperalgesia térmica da cauda. Além disso, substâncias reativas com ácido tiobarbitúrico (TBARS), glutatião reduzido (GSH) e níveis de cálcio total foram estimados para acessar as alterações bioquímicas no tecido do nervo ciático. Alterações histopatológicas foram também observadas no tecido do nervo ciático. O extrato etanólico das folhas de Butea monosperma e pregabalina (servindo de controle positivo) foram administrados por 14 dias consecutivos, iniciando-se no dia da cirurgia. CCI resultou em alterações significativas nos parâmetros comportamentais e bioquímicos. Pretratamento com Butea monosperma atenuou o desenvolvimento das alterações comportamentais, bioquímicas e histopatológicas induzidas pela CCI de maneira dose dependente, comparável ao grupo pretratado com pregabalina. Esses resultados podem ser atribuídos ao potencial antioxidativo, neuroprotetor e às ações modulatórias de canais de cálcio da Butea monosperma
Disciplines: Química,
Medicina
Keyword: Farmacología,
Medicina alternativa,
Neurología,
Dolor neuropático,
Analgésicos,
Herbolaria,
Antioxidantes,
Butea monosperma,
Fabaceae
Keyword: Chemistry,
Medicine,
Alternative medicine,
Neurology,
Pharmacology,
Neuropathic pain,
Analgesics,
Herbist,
Antioxidants,
Butea monosperma,
Fabaceae
Full text: Texto completo (Ver HTML)