| Journal: | Anais da Academia Brasileira de Ciencias |
| Database: | PERIÓDICA |
| System number: | 000408956 |
| ISSN: | 0001-3765 |
| Authors: | Santos, Neyliane F.G. dos1 Silva, Rafael F2 Pinto, Marcela M.P.L1 Silva, Edvane B. da1 Tasat, Deborah R3 Amaral, Ademir1 |
| Institutions: | 1Universidade Federal de Pernambuco, Departamento de Energia Nuclear, Recife, Pernambuco. Brasil 2Universidade de Pernambuco, Departamento de Ciencias Naturais e Exatas, Garanhuns, Pernambuco. Brasil 3Universidad Nacional de San Martín, San Martín, Buenos Aires. Argentina |
| Year: | 2017 |
| Season: | May |
| Volumen: | 89 |
| Pages: | 649-659 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Nota breve o noticia |
| Approach: | Analítico, descriptivo |
| English abstract | Several molecules and events involved in cell response to radiation-induced damage have been investigated towards a personalized radiotherapy. Considering the importance of active caspase-3 in the proteolytic cascade that ensures radiation-induced apoptosis execution, this research was designed to evaluate the expression levels of this protein as a bioindicator of individual radiosensitivity. Peripheral blood samples of 10 healthy individuals were gamma-irradiated (cobalt-60 source) with 1, 2 and 4 Gy (control: non-irradiated samples), and active caspase-3 expression levels were measured in lymphocytes, by flow cytometry, ex vivo and after different times of in vitro incubation (24, 48 and 72 hours). Short-term incubation of 24 h was the most adequate condition to evidence correlations between dose radiation and active caspase-3 expression. For each radiation dose, it was observed a significant inter-individual variation in active caspase-3 expression intensity, suggesting that this parameter may be suitable for evidence individual radiosensitivity. The methodology presented and discussed in this work may help to predict healthy tissues response to radiation exposure toward the better patient outcome |
| Disciplines: | Biología, Química, Medicina |
| Keyword: | Biología celular, Bioquímica, Terapéutica y rehabilitación, Caspase 3, Apoptosis, Citometría de flujo, Radiación ionizante, Linfocitos, Radiosensibilidad |
| Keyword: | Biology, Chemistry, Medicine, Cell biology, Biochemistry, Therapeutics and rehabilitation, Caspase-3, Apoptosis, Flow cytometry, Ionizing radiation, Lymphocytes, Radiosensitivity |
| Full text: | Texto completo (Ver PDF) |
