Production of emulsifier by a strain of Pseudomonas aeruginosa (C1 LBPVMA-UFAL) using lubricant oil as main carbon source
Document title: Production of emulsifier by a strain of Pseudomonas aeruginosa (C1 LBPVMA-UFAL) using lubricant oil as main carbon source
Journal: Acta scientiarum. Technology
Database: PERIÓDICA
System number: 000343273
ISSN: 1806-2563
Authors: 1
1
1
Institutions: 1Universidade Federal de Alagoas, Instituto de Quimica e Biotecnologia, Maceio, Alagoas. Brasil
Year:
Season: Ene-Mar
Volumen: 32
Number: 1
Pages: 33-36
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract The intention of this work was to evaluate the growth of Pseudomonas aeruginosa (C1 LBPVMA-UFAL) on lubricant oil and verify the production of rhamnolipid biosurfactant. Tests in solid medium containing lubricant oil supplemented with different sources of nitrogen were used to evaluate the growth of P. aeruginosa. The growth medium used for production of rhamnolipid was (g L-1): yeast extract, 0.1; NaCl, 1.0; MgSO4, 0.2; MnSO4.H2O, 0.02; 0.5% lubricant oil (w v-1). The biosurfactant concentration was detected 24h after the inoculation, during the exponential phase, with the maximum value obtained up to 120h, although no drastic alteration of pH had been verified. The emulsifier activity was also first detected after 24h of incubation, with maximum production after 120h. The characterization of the produced biosurfactant was performed through thin layer chromatography, which showed the presence of two spots with Rf values equal to 0.71 and 0.5, revealed by reagents specific to rhamnolipids. These results suggested that two types of rhamnolipidic biosurfactant are produced by the strain of P. aeruginosa in limited conditions of nutrients, able to use lubricant oil as main carbon source. This bacterium, isolated from agro-industrial effluent, showed potential to bioremediation assays of contamination with petrol and his derivates
Portuguese abstract O objetivo deste trabalho foi avaliar o crescimento de Pseudomonas aeruginosa (C1 LBPVMAUFAL) sobre óleo lubrificante e verificar a produção de biosurfactante ramnolipídico. Testes em meio sólido com óleo lubrificante suplementado com diferentes fontes de nitrogênio foram usados para avaliar o crescimento de P. aeruginosa. O meio de crescimento utilizado para a produção de ramnolipídeo foi (g L-1): extrato de levedura, 0,1; NaCl, 1,0; MgSO4, 0,2; MnSO4.H2O, 0,02; 0,5% de óleo lubrificante (p v-1). A concentração de biosurfactante foi detectada 24 h após a inoculação, durante a fase exponencial, com valor máximo obtido a 120h, sem verificar alteração drástica de pH. A atividade emulsificante foi também detectada após 24h de incubação, com máxima produção após 120h. A caracterização do biosurfactante produzido foi avaliada por cromatografia de camada delgada, revelando a presença de duas bandas com valores de Rf iguais a 0,71 e 0,5, revelada por reagentes específicos para ramnolipídeos. Estes resultados sugerem que dois tipos de biosurfactantes ramnolipídicos são produzidos pela linhagem de P. aeruginosa em condições limitadas de nutrientes, sendo capaz de utilizar óleo lubrificante como única fonte de carbono. Esta bactéria, isolada de efluente agroindustrial, revelou potencial para ensaios de biorremediação de contaminação com petróleo e seus derivados
Disciplines: Biología,
Química
Keyword: Bioquímica,
Biotecnología,
Bioproducción,
Biosurfactantes,
Sustratos experimentales,
Pseudomonas aeruginosa,
Efluentes agroindustriales
Keyword: Biology,
Chemistry,
Biochemistry,
Biotechnology,
Bioproduction,
Biosurfactants,
Experimental substrates,
Pseudomonas aeruginosa,
Agroindustrial effluents
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