| Journal: | Acta scientiarum. Biological sciences |
| Database: | PERIÓDICA |
| System number: | 000433508 |
| ISSN: | 1679-9283 |
| Authors: | Rodrigues, José Ariévilo Gurgel1 Benevides, Norma Maria Barros2 Tovar, Ana Maria Freire1 Mourão, Paulo Antônio de Souza1 |
| Institutions: | 1Universidade Federal do Rio de Janeiro, Hospital Universitario Clementino Fraga Filho, Rio de Janeiro. Brasil 2Universidade Federal do Ceara, Departamento de Bioquimica e Biologia Molecular, Fortaleza, Ceara. Brasil |
| Year: | 2019 |
| Volumen: | 41 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | Caulerpa cupressoides produces sulfated polysaccharides (Cc-SPs) with serpin-dependent anticoagulant effect, but their actions on thrombin generation (TG) are unknown. This study aimed to partially characterize Cc-SPs and examine their potential as modulators of TG. Infrared analysis characterized extract containing three ulvan fractions (Cc-SP1, -SP2 and -SP3) separated by DEAE-cellulose chromatography, with differences in the relative proportions of sulfate (10.99-18.38%) and total sugars (46.59-51.12%), without presenting proteins. Charge density patterns and nonSPs varying from 8 to > 100 kDa on agarose and polyacrylamide gel electrophoresis by sequential staining with toluidine blue and stains-all were also confirmed by gel permeation chromatography. The molecular weight of Cc-SP2 was not altered after treatment with 0.4 M HCl up to 5 h. Only Cc-SP2 altered the activated partial thromboplastin time (15 ± 0.3 IU) vs. heparin (193 IU) and abolished at high concentrations (> 4.1 μg) TG by intrinsic pathway in 60-fold diluted human plasma, while at 4.1 μg attenuated TG by 33.87% delaying the lag phase (32 min.) vs. control (28 min.). Cc-SP2 induced concentration-dependent TG in system without cephalin. Heparin abolished TG at 4.15-fold lower amount, but did not stimulate TG. Therefore, Cc-SPs express dual effects on thrombosis in vitro |
| Disciplines: | Biología, Química |
| Keyword: | Algas, Bioquímica, Algas marinas, Glucanos sulfatados, Caracterización molecular, Vías de síntesis, Trombina, Caulerpa cupressoides, Caulerpaceae |
| Keyword: | Algae, Biochemistry, Marine algae, Sulfated glycans, Molecular characterization, Synthetic pathways, Thrombin, Caulerpa cupressoides, Caulerpaceae |
| Full text: | http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/35655/pdf |
