| Journal: | Acta scientiarum. Biological sciences |
| Database: | PERIÓDICA |
| System number: | 000433589 |
| ISSN: | 1679-9283 |
| Authors: | Pinheiro, Marcos Vinícius Marques1 Fontana, Daniele Cristina2 Santos, Jullie dos3 Pretto, Matheus Milani4 Azevedo, Gabrieli Cristina Vitalli de4 Schmidt, Denise4 |
| Institutions: | 1Universidade Federal de Santa Catarina, Laboratorio de Fisiologia do Desenvolvimento e Genetica Vegetal, Florianopolis, Santa Catarina. Brasil 2Universidade de Sao Paulo, Escola Superior de Agricultura "Luiz de Queiroz", Piracicaba, Sao Paulo. Brasil 3Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul. Brasil 4Universidade Federal de Santa Maria, Campus Frederico Westphalen, Frederico Westphalen, Rio Grande do Sul. Brasil |
| Year: | 2019 |
| Volumen: | 41 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | In vitro propagation increases the supply and commercialisation of products of interest. For this, optimising the growing conditions and the composition of the culture medium is crucial to benefit the full development of the plants. Thus, the objective was to evaluate the in vitropropagation of Aloysia triphyllaon different culture media, with varying agar and sucrose concentrations. The experiment was conducted as a completely randomised design, 3×3×3 factorial scheme, with three culture media (MS, JADS and WPM), three sucrose concentrations (8, 10 and 12 g L-1) and three agar concentrations (15, 30 and 45 g L-1), with three replicates each and experimental units composed of one plant per replicate. After 25 days of cultivation, the fresh and dry mass of the plants, numbers of leaves, numbers of nodes, plant lengths, numbers of oxidised leaves, hyperhydricity and acclimatization percentages were evaluated. The WPM medium resulted in a reduced fresh mass, reflecting in the low hyperhydricity observed in the explants, and favoured the acclimatization of the plants. Thus, the WPM medium with sucrose (15 g L-1) and agar (12 g L-1) is recommended as the medium most suitable for the in vitroregeneration of Aloysia triphylla |
| Disciplines: | Biología |
| Keyword: | Fisiología vegetal, Cultivo de tejidos, Propagación in vitro, Aclimatación, Hiperhidricidad, Aloysia triphylla, Verbenaceae |
| Keyword: | Plant physiology, Tissue culture, In vitro propagation, Acclimatization, Hyperhydricity, Aloysia triphylla, Verbenaceae |
| Full text: | http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/47105/751375149057 |
